Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2019 May 23

PMID 31114587

Citations 70

Authors

Tiziano Ingegnere

Francesca Romana Mariotti

Andrea Pelosi

Concetta Quintarelli

Biagio De Angelis

Nicola Tumino

Francesca Besi

Claudia Cantoni

Franco Locatelli

Paola Vacca

Lorenzo Moretta

Affiliations

Soon will be listed here.

Abstract

CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

Citing Articles

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