Gut Dysbiosis in the First-Passed Meconium Microbiomes of Korean Preterm Infants Compared to Full-Term Neonates

Overview

Journal Microorganisms

Specialty Microbiology

Date 2024 Jul 27

PMID 39065040

Authors

Sae Yun Kim

Young-Ah Youn

Affiliations

Soon will be listed here.

Abstract

Since gestational age (GA) is an important factor influencing the presence of specific microbiomes, we aimed to characterize the core microbiomes of preterm infants compared to full-term (FT) infants. This study investigated the differences in microbiota composition between very preterm (VP), moderate-to-late preterm (MLP), and FT neonates by examining the core microbiomes of a large cohort of Korean neonates. Meconium samples from 310 neonates with a GA range of 22-40 weeks were collected, and 16S rRNA analyses were performed; 97 samples were obtained from the FT, 59 from the VP, and 154 from the MLP group. Firmicutes, Bacteroidetes, and Proteobacteria were the phylum-level core microbiomes. Infants born before 37 weeks showed a disruption in the core microbiomes. At the phylum level, the relative abundance of Bacteroidetes was positively ( = 0.177, = 0.002) correlated with GA, while that of Proteobacteria was negatively ( = -0.116, = 0.040) correlated with GA. At the genus level, the relative abundances of and were positively correlated with GA ( = 0.157, = 0.006; = 0.160, = 0.005). The meconium of preterm infants exhibited significantly lower -diversities than that of FT infants. -diversities did not appear to differ between the groups. Overall, these findings underscore the importance of GA in shaping the early gut microbiome.

References

Aguilar-Lopez M, Dinsmoor A, Ho T, Donovan S . A systematic review of the factors influencing microbial colonization of the preterm infant gut. Gut Microbes. 2021; 13(1):1-33. PMC: 8023245. DOI: 10.1080/19490976.2021.1884514. View

Stinson L, Boyce M, Payne M, Keelan J . The Not-so-Sterile Womb: Evidence That the Human Fetus Is Exposed to Bacteria Prior to Birth. Front Microbiol. 2019; 10:1124. PMC: 6558212. DOI: 10.3389/fmicb.2019.01124. View

Callahan B, McMurdie P, Rosen M, Han A, Johnson A, Holmes S . DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016; 13(7):581-3. PMC: 4927377. DOI: 10.1038/nmeth.3869. View

Robertson R, Manges A, Finlay B, Prendergast A . The Human Microbiome and Child Growth - First 1000 Days and Beyond. Trends Microbiol. 2018; 27(2):131-147. DOI: 10.1016/j.tim.2018.09.008. View

Kang H, Kim S, Hwang-Bo S, Yoo I, Seo Y, Oh M . Compositional Differences of Meconium Microbiomes of Preterm and Term Infants, and Infants That Developed Necrotizing Enterocolitis or Feeding Intolerance. Pathogens. 2023; 12(1). PMC: 9863680. DOI: 10.3390/pathogens12010055. View

Most J, Dervis S, Haman F, Adamo K, Redman L . Energy Intake Requirements in Pregnancy. Nutrients. 2019; 11(8). PMC: 6723706. DOI: 10.3390/nu11081812. View

Davis N, Proctor D, Holmes S, Relman D, Callahan B . Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data. Microbiome. 2018; 6(1):226. PMC: 6298009. DOI: 10.1186/s40168-018-0605-2. View

Petersen C, Dai D, Boutin R, Sbihi H, Sears M, Moraes T . A rich meconium metabolome in human infants is associated with early-life gut microbiota composition and reduced allergic sensitization. Cell Rep Med. 2021; 2(5):100260. PMC: 8149367. DOI: 10.1016/j.xcrm.2021.100260. View

Claesson M, Jeffery I, Conde S, Power S, OConnor E, Cusack S . Gut microbiota composition correlates with diet and health in the elderly. Nature. 2012; 488(7410):178-84. DOI: 10.1038/nature11319. View

10.

Yoon B, Romero R, Kim C, Jun J, Gomez R, Choi J . Amniotic fluid interleukin-6: a sensitive test for antenatal diagnosis of acute inflammatory lesions of preterm placenta and prediction of perinatal morbidity. Am J Obstet Gynecol. 1995; 172(3):960-70. DOI: 10.1016/0002-9378(95)90028-4. View

11.

Shao Y, Forster S, Tsaliki E, Vervier K, Strang A, Simpson N . Stunted microbiota and opportunistic pathogen colonization in caesarean-section birth. Nature. 2019; 574(7776):117-121. PMC: 6894937. DOI: 10.1038/s41586-019-1560-1. View

12.

Shin N, Whon T, Bae J . Proteobacteria: microbial signature of dysbiosis in gut microbiota. Trends Biotechnol. 2015; 33(9):496-503. DOI: 10.1016/j.tibtech.2015.06.011. View

13.

Dalby M, Hall L . Recent advances in understanding the neonatal microbiome. F1000Res. 2020; 9. PMC: 7255898. DOI: 10.12688/f1000research.22355.1. View

14.

Korpela K, Blakstad E, Moltu S, Strommen K, Nakstad B, Ronnestad A . Intestinal microbiota development and gestational age in preterm neonates. Sci Rep. 2018; 8(1):2453. PMC: 5802739. DOI: 10.1038/s41598-018-20827-x. View

15.

Shi Y, Guo H, Chen J, Sun G, Ren R, Guo M . Initial meconium microbiome in Chinese neonates delivered naturally or by cesarean section. Sci Rep. 2018; 8(1):3255. PMC: 5818670. DOI: 10.1038/s41598-018-21657-7. View

16.

Hill C, Lynch D, Murphy K, Ulaszewska M, Jeffery I, OShea C . Evolution of gut microbiota composition from birth to 24 weeks in the INFANTMET Cohort. Microbiome. 2017; 5(1):4. PMC: 5240274. DOI: 10.1186/s40168-016-0213-y. View

17.

Rinninella E, Raoul P, Cintoni M, Franceschi F, Miggiano G, Gasbarrini A . What is the Healthy Gut Microbiota Composition? A Changing Ecosystem across Age, Environment, Diet, and Diseases. Microorganisms. 2019; 7(1). PMC: 6351938. DOI: 10.3390/microorganisms7010014. View

18.

Jobe A, Bancalari E . Bronchopulmonary dysplasia. Am J Respir Crit Care Med. 2001; 163(7):1723-9. DOI: 10.1164/ajrccm.163.7.2011060. View

19.

Olm M, Bhattacharya N, Crits-Christoph A, Firek B, Baker R, Song Y . Necrotizing enterocolitis is preceded by increased gut bacterial replication, , and fimbriae-encoding bacteria. Sci Adv. 2019; 5(12):eaax5727. PMC: 6905865. DOI: 10.1126/sciadv.aax5727. View

20.

Morais J, Marques C, Teixeira D, Durao C, Faria A, Brito S . Extremely preterm neonates have more in meconium than very preterm neonates - the microbial colonization hypothesis. Gut Microbes. 2020; 12(1):1785804. PMC: 7524394. DOI: 10.1080/19490976.2020.1785804. View