The Potential of a New Natural Vessel Source: Decellularized Intercostal Arteries As Sufficiently Long Small-Diameter Vascular Grafts

Overview

Journal Bioengineering (Basel)

Date 2024 Jul 27

PMID 39061783

Authors

Yuan Xia

Haiyun Zhou

Jing-Song Ou

Yunqi Liu

Affiliations

Soon will be listed here.

Abstract

Small-diameter vascular grafts (SDVGs) are severely lacking in clinical settings. Therefore, our study investigates a new source of biological vessels-bovine and porcine decellularized intercostal arteries (DIAs)-as potential SDVGs. We utilized a combination of SDS and Triton X-100 to perfuse the DIAs, establishing two different time protocols. The results show that perfusing with 1% concentrations of each decellularizing agent for 48 h yields DIAs with excellent biocompatibility and mechanical properties. The porcine decellularized intercostal arteries (PDIAs) we obtained had a length of approximately 14 cm and a diameter of about 1.5 mm, while the bovine decellularized intercostal arteries (BDIAs) were about 29 cm long with a diameter of approximately 2.2 mm. Although the lengths and diameters of both the PDIAs and BDIAs are suited for coronary artery bypass grafting (CABG), as the typical diameter of autologous arteries used in CABG is about 2 mm and the grafts required are at least 10 cm long, our research indicates that BDIAs possess more ideal mechanical characteristics for CABG than PDIAs, showing significant potential. Further enhancements may be necessary to address their limited hemocompatibility.

References

Hiob M, She S, Muiznieks L, Weiss A . Biomaterials and Modifications in the Development of Small-Diameter Vascular Grafts. ACS Biomater Sci Eng. 2021; 3(5):712-723. DOI: 10.1021/acsbiomaterials.6b00220. View

Cai Z, Gu Y, Cheng J, Li J, Xu Z, Xing Y . Decellularization, cross-linking and heparin immobilization of porcine carotid arteries for tissue engineering vascular grafts. Cell Tissue Bank. 2019; 20(4):569-578. DOI: 10.1007/s10561-019-09792-5. View

Campbell E, Cahill P, Lally C . Investigation of a small-diameter decellularised artery as a potential scaffold for vascular tissue engineering; biomechanical evaluation and preliminary cell seeding. J Mech Behav Biomed Mater. 2012; 14:130-42. DOI: 10.1016/j.jmbbm.2012.06.001. View

Dahl S, Koh J, Prabhakar V, Niklason L . Decellularized native and engineered arterial scaffolds for transplantation. Cell Transplant. 2003; 12(6):659-66. View

Nie N, Tu Q, Wang J, Chao F, Liu R, Zhang Y . Synthesis of copolymers using dendronized polyethylene glycol and assay of their blood compatibility and antibacterial adhesion activity. Colloids Surf B Biointerfaces. 2012; 97:226-35. DOI: 10.1016/j.colsurfb.2012.04.012. View

Eufrasio-da-Silva T, Ruiz-Hernandez E, ODwyer J, Picazo-Frutos D, Duffy G, Murphy B . Enhancing medial layer recellularization of tissue-engineered blood vessels using radial microchannels. Regen Med. 2019; 14(11):1013-1028. DOI: 10.2217/rme-2019-0011. View

Kang B, Li H, Chen Y, Deng M, Li Y, Peng Y . High-density lipoprotein regulates angiogenesis by affecting autophagy via miRNA-181a-5p. Sci China Life Sci. 2023; 67(2):286-300. DOI: 10.1007/s11427-022-2381-7. View

Liu Y, Chen C, Xie X, Yuan H, Tang Z, Qian T . Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft . Front Bioeng Biotechnol. 2022; 10:816513. PMC: 8987116. DOI: 10.3389/fbioe.2022.816513. View

Zhang J, Hu Z, Turner N, Teng S, Cheng W, Zhou H . Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template. Biomaterials. 2016; 89:114-26. DOI: 10.1016/j.biomaterials.2016.02.040. View

10.

Li Y, Zhou Y, Qiao W, Shi J, Qiu X, Dong N . Application of decellularized vascular matrix in small-diameter vascular grafts. Front Bioeng Biotechnol. 2023; 10:1081233. PMC: 9852870. DOI: 10.3389/fbioe.2022.1081233. View

11.

Fukui S, Fukuda H, Toda K, Yoshitatsu M, Funatsu T, Masai T . Remodeling of the radial artery anastomosed to the internal thoracic artery as a composite straight graft. J Thorac Cardiovasc Surg. 2007; 134(5):1136-42. DOI: 10.1016/j.jtcvs.2007.07.024. View

12.

Schneider K, Rohringer S, Kapeller B, Grasl C, Kiss H, Heber S . Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts. Acta Biomater. 2020; 116:246-258. DOI: 10.1016/j.actbio.2020.08.037. View

13.

Boccafoschi F, Botta M, Fusaro L, Copes F, Ramella M, Cannas M . Decellularized biological matrices: an interesting approach for cardiovascular tissue repair and regeneration. J Tissue Eng Regen Med. 2015; 11(5):1648-1657. DOI: 10.1002/term.2103. View

14.

Dahan N, Zarbiv G, Sarig U, Karram T, Hoffman A, Machluf M . Porcine small diameter arterial extracellular matrix supports endothelium formation and media remodeling forming a promising vascular engineered biograft. Tissue Eng Part A. 2011; 18(3-4):411-22. DOI: 10.1089/ten.TEA.2011.0173. View

15.

Ohtani N, Kiyokawa K, Asada H, Kawakami T, Haga M, Akasaka N . Evaluation of an internal thoracic artery as a coronary artery bypass graft by intercostal duplex scanning ultrasonography. Jpn J Thorac Cardiovasc Surg. 2001; 49(6):343-6. DOI: 10.1007/BF02913147. View

16.

Matsuda T, Ito S . Surface coating of hydrophilic-hydrophobic block co-polymers on a poly(acrylonitrile) haemodialyser reduces platelet adhesion and its transmembrane stimulation. Biomaterials. 1994; 15(6):417-22. DOI: 10.1016/0142-9612(94)90219-4. View

17.

Gui L, Muto A, Chan S, Breuer C, Niklason L . Development of decellularized human umbilical arteries as small-diameter vascular grafts. Tissue Eng Part A. 2009; 15(9):2665-76. PMC: 2735599. DOI: 10.1089/ten.tea.2008.0526. View

18.

Cai Z, Gu Y, Xiao Y, Wang C, Wang Z . Porcine carotid arteries decellularized with a suitable concentration combination of Triton X-100 and sodium dodecyl sulfate for tissue engineering vascular grafts. Cell Tissue Bank. 2020; 22(2):277-286. DOI: 10.1007/s10561-020-09876-7. View

19.

Guyette J, Gilpin S, Charest J, Tapias L, Ren X, Ott H . Perfusion decellularization of whole organs. Nat Protoc. 2014; 9(6):1451-68. DOI: 10.1038/nprot.2014.097. View

20.

Herrmann F, Lamm P, Wellmann P, Milz S, Hagl C, Juchem G . Autologous endothelialized vein allografts in coronary artery bypass surgery - Long term results. Biomaterials. 2019; 212:87-97. DOI: 10.1016/j.biomaterials.2019.05.019. View