Toxin Production and Haemagglutination in Strains of Escherichia Coli from Diarrhoea in Brescia, Italy

Overview

Journal J Hyg (Lond)

Publisher Cambridge University Press

Specialty Public Health

Date 1985 Oct 1

PMID 3905955

Citations 17

Authors

R Bisicchia

R Ciammarughi

A Caprioli

V Falbo

F M Ruggeri

Affiliations

Soon will be listed here.

Abstract

Two hundred and ninety-nine different strains of Escherichia coli, isolated from 172 patients with diarrhoea and 113 healthy subjects, were examined for enterotoxin, cytotoxin and haemolysin (Hly) production and for mannose-resistant haemagglutination (MRHA) and invasive properties. Three strains proved enterotoxigenic, none enteroinvasive; cytotoxin and Hly production was shown in 25 strains from patients and in 3 from controls. Ten strains produced the cytotoxic necrotizing factor (CNF), 6 released other factors which kill cell cultures. Hly production was shown in 21 strains, 9 of which were also positive for CNF. MRHA was detected in 26% of strains from diarrhoea compared with 14% of strains from healthy people. A strong association between toxin production and MRHA was demonstrated. Serotyping results showed that the strains exhibiting virulence traits mostly belonged to serogroups commonly involved in extra-intestinal infections. The possible role of strains of E. coli showing one or more virulence factors as opportunistic pathogens in diarrhoeal diseases is discussed.

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