Comparison of Different Hydroxyapatite Composites for Bone Tissue Repair: and Analyses

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2024 Jul 26

PMID 39055877

Authors

Xiaoyu Su

Xiang Si

Yuyang Liu

Nana Xiong

Siyuan Li

Lu Tang

Zheng Shi

Lijia Cheng

Fei Zhang

Affiliations

Soon will be listed here.

Abstract

Objectives: The material used for bone tissue repair needs to be simultaneously osteoconductive, osteoinductive, and osteogenic. To overcome this problem, researchers combine hydroxyapatite (HA) with natural materials to improve properties. This paper compares the effects of angiogenesis and osteogenesis with different composites through experiments and characterization analysis.

Materials And Methods: Chitosan/nHA (CS/nHA) and sodium alginate/nHA (SA/nHA) microspheres were synthesized via reverse-phase emulsification crosslinking and analyzed using scanning electron microscopy (SEM), energy dispersion spectroscopy (EDS), and X-ray diffraction (XRD). Implanted into mouse thigh muscles, their angiogenic and osteogenic potentials were assessed after 8 and 12 weeks through various staining methods and immunohistochemistry.

Results: The mean vascular density (MVD) of CS/nHA, CaP/nHA, and SA/nHA groups was (134.92±35.30) n/mm, (159.09±22.14) n/mm, (160.31±42.23) n/mm at 12 weeks, respectively. The MVD of the CaP/nHA and SA/nHA groups were significantly higher than that of the CS/nHA group. The collagen volume fractions (CVF) were 34.13%, 51.53%, and 54.96% in the CS/nHA, CaP/nHA, and SA/nHA groups, respectively. In addition, the positive expression area ratios of OPN and CD31 in the CaP/nHA and SA/nHA groups were also significantly higher than those in the CS/nHA group.

Conclusion: The ability of SA/nHA composite microspheres in osteogenesis and angiogenesis is clearly superior to that of the CS/nHA group and is comparable to that of CaP/nHA, which has superior osteogenesis ability, indicating that SA/nHA composite microspheres have greater application prospects in bone tissue engineering.

References

Wu X, Stroll S, Lantigua D, Suvarnapathaki S, Camci-Unal G . Eggshell particle-reinforced hydrogels for bone tissue engineering: an orthogonal approach. Biomater Sci. 2019; 7(7):2675-2685. DOI: 10.1039/c9bm00230h. View

Li M, Bai Y, Pan X, Wang J, Chen W, Luo J . [Study on the correlation between the content of bone morphogenetic protein 2 in demineralized bone matrix and its osteogenic activity and ]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2021; 35(5):620-626. PMC: 8175199. DOI: 10.7507/1002-1892.202012006. View

Pravdyuk A, Petrenko Y, Fuller B, Petrenko A . Cryopreservation of alginate encapsulated mesenchymal stromal cells. Cryobiology. 2013; 66(3):215-22. DOI: 10.1016/j.cryobiol.2013.02.002. View

Lim K, Patel D, Dutta S, Choung H, Jin H, Bhattacharjee A . Human Teeth-Derived Bioceramics for Improved Bone Regeneration. Nanomaterials (Basel). 2020; 10(12). PMC: 7761315. DOI: 10.3390/nano10122396. View

Sohn H, Oh J . Review of bone graft and bone substitutes with an emphasis on fracture surgeries. Biomater Res. 2019; 23:9. PMC: 6417250. DOI: 10.1186/s40824-019-0157-y. View

Schmidt A . Autologous bone graft: Is it still the gold standard?. Injury. 2021; 52 Suppl 2:S18-S22. DOI: 10.1016/j.injury.2021.01.043. View

He Y, Tian Y, Zhang W, Wang X, Yang X, Li B . Fabrication of oxidized sodium alginate-collagen heterogeneous bilayer barrier membrane with osteogenesis-promoting ability. Int J Biol Macromol. 2022; 202:55-67. DOI: 10.1016/j.ijbiomac.2021.12.155. View

Khalaf A, Wei Y, Wan J, Zhu J, Peng Y, Abdul Kadir S . Bone Tissue Engineering through 3D Bioprinting of Bioceramic Scaffolds: A Review and Update. Life (Basel). 2022; 12(6). PMC: 9225502. DOI: 10.3390/life12060903. View

Chircov C, Miclea I, Grumezescu V, Grumezescu A . Essential Oils for Bone Repair and Regeneration-Mechanisms and Applications. Materials (Basel). 2021; 14(8). PMC: 8069393. DOI: 10.3390/ma14081867. View

10.

G V Y, Nagendra A, P S, Chatterjee K, Venkatesan J . Fucoidan-Incorporated Composite Scaffold Stimulates Osteogenic Differentiation of Mesenchymal Stem Cells for Bone Tissue Engineering. Mar Drugs. 2022; 20(10). PMC: 9604642. DOI: 10.3390/md20100589. View

11.

Ressler A . Chitosan-Based Biomaterials for Bone Tissue Engineering Applications: A Short Review. Polymers (Basel). 2022; 14(16). PMC: 9416295. DOI: 10.3390/polym14163430. View

12.

Li Z, Ramay H, Hauch K, Xiao D, Zhang M . Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials. 2005; 26(18):3919-28. DOI: 10.1016/j.biomaterials.2004.09.062. View

13.

Liang T, Wu J, Li F, Huang Z, Pi Y, Miao G . Drug-loading three-dimensional scaffolds based on hydroxyapatite-sodium alginate for bone regeneration. J Biomed Mater Res A. 2020; 109(2):219-231. DOI: 10.1002/jbm.a.37018. View

14.

Steijvers E, Ghei A, Xia Z . Manufacturing artificial bone allografts: a perspective. Biomater Transl. 2022; 3(1):65-80. PMC: 9255790. DOI: 10.12336/biomatertransl.2022.01.007. View

15.

Chatzipetros E, Damaskos S, Tosios K, Christopoulos P, Donta C, Kalogirou E . The effect of nano-hydroxyapatite/chitosan scaffolds on rat calvarial defects for bone regeneration. Int J Implant Dent. 2021; 7(1):40. PMC: 8141479. DOI: 10.1186/s40729-021-00327-w. View

16.

Yi M, Nie Y, Zhang C, Shen B . Application of Mesoporous Silica Nanoparticle-Chitosan-Loaded BMP-2 in the Repair of Bone Defect in Chronic Osteomyelitis. J Immunol Res. 2022; 2022:4450196. PMC: 9357812. DOI: 10.1155/2022/4450196. View

17.

Park J, Lee E, Knowles J, Kim H . Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration. J Biomater Appl. 2013; 28(7):1079-84. PMC: 4107800. DOI: 10.1177/0885328213496486. View

18.

Luo Y, Lode A, Wu C, Chang J, Gelinsky M . Alginate/nanohydroxyapatite scaffolds with designed core/shell structures fabricated by 3D plotting and in situ mineralization for bone tissue engineering. ACS Appl Mater Interfaces. 2015; 7(12):6541-9. DOI: 10.1021/am508469h. View

19.

Maghsoudlou M, Nassireslami E, Saber-Samandari S, Khandan A . Bone Regeneration Using Bio-Nanocomposite Tissue Reinforced with Bioactive Nanoparticles for Femoral Defect Applications in Medicine. Avicenna J Med Biotechnol. 2020; 12(2):68-76. PMC: 7229459. View

20.

Bagherifard A, Joneidi Yekta H, Akbari Aghdam H, Motififard M, Sanatizadeh E, Ghadiri Nejad M . Improvement in osseointegration of tricalcium phosphate-zircon for orthopedic applications: an in vitro and in vivo evaluation. Med Biol Eng Comput. 2020; 58(8):1681-1693. DOI: 10.1007/s11517-020-02157-1. View