A New Application of Avocado Oil to Enrich the Biological Activities of Polycaprolactone Membranes for Tissue Engineering

Overview

Journal Biopolymers

Publisher Wiley

Date 2024 Jul 20

PMID 39032016

Authors

Merve Capkin Yurtsever

Selin Aydogan

Zeynep Iyigundogdu

Alican Comertpay

Didem Demir

Seda Ceylan

Affiliations

Soon will be listed here.

Abstract

The metabolites synthesized by plants to protect themselves serves as natural antimicrobial agents used in biomaterials. In this study, avocado oil (AO), was incorporated as a plant source and natural antimicrobial agent into polycaprolactone (PCL) membranes. The effects of varying AO ratios (25, 50, and 100 wt%.-PCL@25AO, PCL@50AO, PCL@100AO) on PCL membrane morphology, chemical structure, wettability, antimicrobial activity, and cell viabilities were investigated. It was demonstrated that the AO acts as a pore-forming agent in solvent-casted membranes. Young's modulus of the membranes varied between 602.68 and 31.92 MPa and more flexible membranes were obtained with increasing AO content. Inhibition zones of AO were recorded between 7.86 and 13.97 mm against clinically relevant microbial strains including bacteria, yeast, and fungi. Antimicrobial activity of AO was retained in PCL membranes at all ratios. Resazurin assay indicated that PCL@25AO membranes were cytocompatible with mouse fibroblast cells (L929 cell line) on day 6 showing 72.4% cell viability with respect to neat PCL membranes. Viability results were supported by scanning electron microscopy images and DAPI staining. The overall results of this study highlight the potential of PCL@25AO membranes as a biomaterial with antimicrobial properties, cytocompatibility, and mechanical strength suitable for various biomedical applications.

Citing Articles

A new application of avocado oil to enrich the biological activities of polycaprolactone membranes for tissue engineering.

Yurtsever M, Aydogan S, Iyigundogdu Z, Comertpay A, Demir D, Ceylan S Biopolymers. 2024; 115(6):e23617.

PMID: 39032016 PMC: 11579220. DOI: 10.1002/bip.23617.

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