OpenTn5: Open-Source Resource for Robust and Scalable Tn5 Transposase Purification and Characterization

Overview

Journal bioRxiv

Date 2024 Jul 19

PMID 39026714

Authors

Jan Soroczynski

Lauren J Anderson

Joanna L Yeung

Justin M Rendleman

Deena A Oren

Hide A Konishi

Viviana I Risca

Affiliations

Soon will be listed here.

Abstract

Tagmentation combines DNA fragmentation and sequencing adapter addition by leveraging the transposition activity of the bacterial cut-and-paste Tn5 transposase, to enable efficient sequencing library preparation. Here we present an open-source protocol for the generation of multi-purpose hyperactive Tn5 transposase, including its benchmarking in CUT&Tag, bulk and single-cell ATAC-seq. The OpenTn5 protocol yields multi-milligram quantities of pG-Tn5 protein per liter of culture, sufficient for thousands of tagmentation reactions and the enzyme retains activity in storage for more than a year.

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