Conversion of Mouse-Derived Hybridomas to Tasmanian Devil Recombinant IgG Antibodies

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Jul 17

PMID 39017897

Authors

Bailey Slyp

Jocelyn M Darby

Andrew S Flies

Affiliations

Soon will be listed here.

Abstract

The hybridoma method for production of monoclonal antibodies has been a cornerstone of biomedical research for several decades. Here we convert the monoclonal antibody sequence from mouse-derived hybridomas into a "devilized" recombinant antibody with devil IgG heavy chain and IgK light chain. The chimeric recombinant antibody can be used in functional assays, immunotherapy, and to improve understanding of antibodies and Fc receptors in Tasmanian devils. The process can be readily modified for other species.

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