Large Extracellular Vesicles from Induced Pluripotent Stem Cell-marrow Stem Cells Enhance Limb Angiogenesis Via ERK/MAPK

Overview

Journal Nanomedicine (Lond)

Specialty Biotechnology

Date 2024 Jul 16

PMID 39012207

Authors

Ziyu Huang

Zhengsheng Chen

Teng Ye

Lei Luo

Juntao Zhang

Qing Li

Yang Wang

Bizeng Zhao

Affiliations

Soon will be listed here.

Abstract

This study aims to investigate the effects of large extracellular vesicles (EVs) induced by pluripotent stem cell-derived mesenchymal stem cells on lower limb ischemic disease and explore its potential mechanisms. The pathology of muscles was accessed by H&E staining and immunofluorescence staining. , we conducted wound-healing assay, tube formation assay, RT qPCR, ELISA, RNA sequencing and proteomic analysis. iMSCs-lEVs alleviated the injury of ischemic lower limb and promoted the recovery of lower limb function. , iMSCs-lEVs promoted the proliferation, migration, and angiogenesis of HMEC-1 cells by regulating the ERK/MAPK signing pathway. This study demonstrated that iMSCs-lEVs promoted endothelial cell angiogenesis via the ERK/MAPK signaling pathway, thereby improving function after lower limb ischemic injury.

Citing Articles

Emerging Strategies for Revascularization: Use of Cell-Derived Extracellular Vesicles and Artificial Nanovesicles in Critical Limb Ischemia.

Ravi Mythili V, Rajendran R, Arun R, Loganathbabu V, Reyaz D, Nagarajan A Bioengineering (Basel). 2025; 12(1).

PMID: 39851366 PMC: 11762151. DOI: 10.3390/bioengineering12010092.

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