Dysfunction of the Murine Liver with Aging and Its Improvement with the Continuous Consumption of EC-12

Overview

Journal Nutrients

Date 2024 Jul 13

PMID 38999780

Authors

Yuko Makioka-Itaya

Ryo Inoue

Takamitsu Tsukahara

Affiliations

Soon will be listed here.

Abstract

Chronic inflammation is involved in the development of age-related diseases. Given its persistence, controlling chronic inflammation is essential for preventing age-related diseases. In this study, we investigated the effects of EC-12 (EC-12), which has immunomodulatory and antioxidant effects, on liver gene expression and aging phenomena in mice. Short-term EC-12 administration stimulated the expression of genes involved in lipid synthesis and metabolism in the liver. Furthermore, long-term EC-12 administration from 10 weeks to 1.5 years of age resulted in significant increases in blood interleukin (IL)-6 and IL-10 concentrations (both < 0.05) and a significant decrease in the monocyte chemotactic protein-1 concentration ( < 0.05). These results indicated pathologic improvement, such as suppression of fat degeneration in the liver. These results suggest that continuous EC-12 intake from a young age can suppress liver function abnormalities, which is one of the aging phenomena in old age, and contribute to health in old age.

References

Franceschi C, Campisi J . Chronic inflammation (inflammaging) and its potential contribution to age-associated diseases. J Gerontol A Biol Sci Med Sci. 2014; 69 Suppl 1:S4-9. DOI: 10.1093/gerona/glu057. View

Dziedzic T . Systemic inflammatory markers and risk of dementia. Am J Alzheimers Dis Other Demen. 2006; 21(4):258-62. PMC: 10833275. DOI: 10.1177/1533317506289260. View

Tanaka Y, Hirose Y, Yamamoto Y, Yoshikai Y, Murosaki S . Daily intake of heat-killed Lactobacillus plantarum L-137 improves inflammation and lipid metabolism in overweight healthy adults: a randomized-controlled trial. Eur J Nutr. 2019; 59(6):2641-2649. PMC: 7413902. DOI: 10.1007/s00394-019-02112-3. View

Simon A, Hollander G, McMichael A . Evolution of the immune system in humans from infancy to old age. Proc Biol Sci. 2015; 282(1821):20143085. PMC: 4707740. DOI: 10.1098/rspb.2014.3085. View

Tateda K, Matsumoto T, Miyazaki S, Yamaguchi K . Lipopolysaccharide-induced lethality and cytokine production in aged mice. Infect Immun. 1996; 64(3):769-74. PMC: 173836. DOI: 10.1128/iai.64.3.769-774.1996. View

Ahmadi S, Wang S, Nagpal R, Wang B, Jain S, Razazan A . A human-origin probiotic cocktail ameliorates aging-related leaky gut and inflammation via modulating the microbiota/taurine/tight junction axis. JCI Insight. 2020; 5(9). PMC: 7253024. DOI: 10.1172/jci.insight.132055. View

Gotoh K, Inoue M, Masaki T, Chiba S, Shimasaki T, Ando H . A novel anti-inflammatory role for spleen-derived interleukin-10 in obesity-induced inflammation in white adipose tissue and liver. Diabetes. 2012; 61(8):1994-2003. PMC: 3402315. DOI: 10.2337/db11-1688. View

Furman D, Campisi J, Verdin E, Carrera-Bastos P, Targ S, Franceschi C . Chronic inflammation in the etiology of disease across the life span. Nat Med. 2019; 25(12):1822-1832. PMC: 7147972. DOI: 10.1038/s41591-019-0675-0. View

Timper K, Denson J, Steculorum S, Heilinger C, Engstrom-Ruud L, Wunderlich C . IL-6 Improves Energy and Glucose Homeostasis in Obesity via Enhanced Central IL-6 trans-Signaling. Cell Rep. 2017; 19(2):267-280. DOI: 10.1016/j.celrep.2017.03.043. View

10.

Rezzani R, Franco C . Liver, Oxidative Stress and Metabolic Syndromes. Nutrients. 2021; 13(2). PMC: 7909777. DOI: 10.3390/nu13020301. View

11.

Fukushima Y, Miyaguchi S, Yamano T, Kaburagi T, Iino H, Ushida K . Improvement of nutritional status and incidence of infection in hospitalised, enterally fed elderly by feeding of fermented milk containing probiotic Lactobacillus johnsonii La1 (NCC533). Br J Nutr. 2007; 98(5):969-77. DOI: 10.1017/S0007114507764723. View

12.

Tamaki R, Takahashi M, Tai S, Makioka-Itaya Y, Ijich T, Inoue R . Exploratory investigation of the anti-inflammatory effects of RNase A-treated strain EC-12. Biosci Biotechnol Biochem. 2019; 83(7):1343-1353. DOI: 10.1080/09168451.2019.1608805. View

13.

Inoue R, Tsukahara T, Matsukawa N, Watanabe T, Bukawa W, Nakayama K . Rapid induction of an immune response in rat Peyer's patch after oral administration of Enterococcus faecalis strain EC-12. Biosci Biotechnol Biochem. 2013; 77(4):863-6. DOI: 10.1271/bbb.120838. View

14.

Nguyen H, Gu M, Werlinger P, Cho J, Cheng J, Suh J . MJM60958 as a Potential Probiotic Alleviated Non-Alcoholic Fatty Liver Disease in Mice Fed a High-Fat Diet by Modulating Lipid Metabolism, Inflammation, and Gut Microbiota. Int J Mol Sci. 2022; 23(21). PMC: 9658623. DOI: 10.3390/ijms232113436. View

15.

Rinella M, Lazarus J, Ratziu V, Francque S, Sanyal A, Kanwal F . A multisociety Delphi consensus statement on new fatty liver disease nomenclature. J Hepatol. 2023; 79(6):1542-1556. DOI: 10.1016/j.jhep.2023.06.003. View

16.

Agita A, Alsagaff M . Inflammation, Immunity, and Hypertension. Acta Med Indones. 2017; 49(2):158-165. View

17.

Huang S, Chen L, Wang M, Hsu C, Chan C, Li J . PS23 Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice. Nutrients. 2018; 10(7). PMC: 6073302. DOI: 10.3390/nu10070894. View

18.

Fiorucci S, Biagioli M, Zampella A, Distrutti E . Bile Acids Activated Receptors Regulate Innate Immunity. Front Immunol. 2018; 9:1853. PMC: 6099188. DOI: 10.3389/fimmu.2018.01853. View

19.

Robinson M, Harmon C, OFarrelly C . Liver immunology and its role in inflammation and homeostasis. Cell Mol Immunol. 2016; 13(3):267-76. PMC: 4856809. DOI: 10.1038/cmi.2016.3. View

20.

de Moreno de LeBlanc A, Chaves S, Carmuega E, Weill R, Antoine J, Perdigon G . Effect of long-term continuous consumption of fermented milk containing probiotic bacteria on mucosal immunity and the activity of peritoneal macrophages. Immunobiology. 2008; 213(2):97-108. DOI: 10.1016/j.imbio.2007.07.002. View