Studies on B-cell Memory. II. T-cell Independent Antigen Can Induce B-cell Memory

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1979 Oct 1

PMID 389783

Citations 12

Authors

T Hosokawa

Affiliations

Soon will be listed here.

Abstract

Both athymic nude mice and normal mice primed with a T-cell independent antigen, i.e. dinitrophenylated dextran (DNP-DE), at a sub-immunogenic dose, produced very poor anti-DNP responses to a later challenge with the same antigen. B-cell memory was expressed, however, as an enhanced IgM response after the challenge of the DNP-DE-primed mice with the T-cell dependent antigen (dinitrophenylated haemocyanin, DNP-KLH) in the presence of functional T-cells. Moreover, DNP-DE-primed spleen cells also revealed an enhanced IgM response after adoptive transfer into irradiated recipients and challenge with DNP-DE. The injections of DNP-DE-primed nude mouse serum into unprimed mice resulted in the reduction of anti-DNP response to the immunization with DNP-DE. These results indicate that (a) T-cell independent DNP-DE causes the differentiation of B cells not only into antibody-forming cells but also into memory cells, (b) these memory cells can be triggered in situ by the T-cell dependent DNP-KLH in the presence of helper T cells but not by T-cell independent antigen, and (c) some humoral factor(s) induced by DNP-DE-priming seems to interfere with the expression of B-cell memory only when challenged with T-cell independent DNP-DE.

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References

Eisen H, Kern M, NEWTON W, Helmreich E . A study of the distribution of 2,4-dinitrobenzene sensitizers between isolated lymph node cells and extracellular medium in relation to induction of contact skin sensitivity. J Exp Med. 1959; 110(2):187-206. PMC: 2136994. DOI: 10.1084/jem.110.2.187. View

Quintans J, Cosenza H . Antibody response to phosphorylcholine in vitro. II. Analysis of T-dependent and T-independent responses. Eur J Immunol. 1976; 6(6):399-405. DOI: 10.1002/eji.1830060605. View

Klaus G, Humphrey J . B cell tolerance induced by polymeric antigens. I. Comparison of the dose and epitope density requirements for inactivation of primed and unprimed B cells in vivo. Eur J Immunol. 1976; 5(6):361-5. DOI: 10.1002/eji.1830050602. View

Hosokawa T, Amagai T, Muramatsu S . Studies on B-cell memory. I. Generation and exhaustion of B-cell memory by thymus-dependent antigen in T-cell depleted mice. Immunology. 1979; 38(2):283-9. PMC: 1457936. View

Diaz-Espada F, Bernabe R . Development of B cell subsets: effect of priming on in vitro TNP-LPS responsiveness. J Immunol. 1978; 121(1):13-8. View

Braley-Mullen H . Antigen requirements for induction of B-memory cells: studies with dinitrophenyl coupled to T-dependent and T-independent carriers. J Exp Med. 1978; 147(6):1824-31. PMC: 2184318. DOI: 10.1084/jem.147.6.1824. View

Inaba K, Nakano K, Muramatsu S . Regulatory function of T lymphocytes in the immune response to polyvinyl pyrrolidone. I. Two categories of suppressor T cells. Cell Immunol. 1978; 39(2):260-75. DOI: 10.1016/0008-8749(78)90102-8. View

Takaoki M, Kawaguchi S, Katsura Y, Muramatsu S . Transition in the character of immunological memory in mice after immunization. I. Memory for IgM and IgG antibody responses. Jpn J Microbiol. 1976; 20(4):255-61. DOI: 10.1111/j.1348-0421.1976.tb00986.x. View

JENNINGS J, RITTENBERG M . Evidence for separate subpopulations of B cells responding to T-independent and T-dependent immunogens. J Immunol. 1976; 117(5 Pt.2):1749-52. View

10.

Desaymard C, Howard J . Role of epitope density in the induction of immunity and tolerance with thymus-independent antigens. II. Studies with 2,4-dinitrophenyl conjugates in vivo. Eur J Immunol. 1975; 5(8):541-5. DOI: 10.1002/eji.1830050807. View

11.

Hosono M, Muramatsu S . Use of 2-mercaptoethanol for distinguishing between IgM and IgG antibody-producing cells of mice immunized with bovine globulin. J Immunol. 1972; 109(4):857-63. View

12.

Playfair J, Purves E . Separate thymus dependent and thymus independent antibody forming cell precursors. Nat New Biol. 1971; 231(22):149-51. DOI: 10.1038/newbio231149a0. View

13.

Goidl E, Siskind G . Ontogeny of B-lymphocyte function. I. Restricted heterogeneity of the antibody response of B lymphocytes from neonatal and fetal mice. J Exp Med. 1974; 140(5):1285-302. PMC: 2139717. DOI: 10.1084/jem.140.5.1285. View

14.

Howard J, Courtenay B . Induction of B cell tolerance to polysaccharides by exhaustive immunization and during immunosuppression with cyclophosphamide. Eur J Immunol. 1974; 4(9):603-8. DOI: 10.1002/eji.1830040905. View

15.

Baker P, STASHAK P, Amsbaugh D, PRESCOTT B . Characterization of the antibody response to type 3 pneumococcal polysaccharide at the cellular level. I. Dose-response studies and the effect of prior immunization on the magnitude of the antibody response. Immunology. 1971; 20(4):469-80. PMC: 1456005. View

16.

Miranda J . Studies on immunological paralysis. IX. The immunogenicity and tolerogenicity of levan (polyfructose) in mice. Immunology. 1972; 23(6):829-42. PMC: 1407990. View

17.

Takemori T, Tada T . Selective roles of thymus-derived lymphocytes in the antibody response. II. Preferential suppression of high-affinity antibody-forming cells by carrier-primed suppressor T cells. J Exp Med. 1974; 140(1):253-66. PMC: 2139704. DOI: 10.1084/jem.140.1.253. View

18.

Andersson B . Studies on the regulation of avidity at the level of the single antibody-forming cell. The effect of antigen dose and time after immunization. J Exp Med. 1970; 132(1):77-88. PMC: 2138750. DOI: 10.1084/jem.132.1.77. View

19.

Fidler J . In vivo immune response to TNP hapten coupled to thymus-independent carrier lipopolysaccharide. Cell Immunol. 1975; 16(2):223-36. DOI: 10.1016/0008-8749(75)90114-8. View

20.

LEWIS G, Ranken R, Nitecki D, Goodman J . Murine B-cell subpopulations responsive to T-dependent and T-independent antigens. J Exp Med. 1976; 144(2):382-97. PMC: 2190386. DOI: 10.1084/jem.144.2.382. View