Competition and Synergy of Arp2/3 and Formins in Nucleating Actin Waves

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2024 Jul 5

PMID 38968072

Authors

Xiang Le Chua

Chee San Tong

Maohan Su

X J Xu

Shengping Xiao

Xudong Wu

Min Wu

Affiliations

Soon will be listed here.

Abstract

Actin assembly and dynamics are crucial for maintaining cell structure and changing physiological states. The broad impact of actin on various cellular processes makes it challenging to dissect the specific role of actin regulatory proteins. Using actin waves that propagate on the cortex of mast cells as a model, we discovered that formins (FMNL1 and mDia3) are recruited before the Arp2/3 complex in actin waves. GTPase Cdc42 interactions drive FMNL1 oscillations, with active Cdc42 and the constitutively active mutant of FMNL1 capable of forming waves on the plasma membrane independently of actin waves. Additionally, the delayed recruitment of Arp2/3 antagonizes FMNL1 and active Cdc42. This antagonism is not due to competition for monomeric actin but rather for their common upstream regulator, active Cdc42, whose levels are negatively regulated by Arp2/3 via SHIP1 recruitment. Collectively, our study highlights the complex feedback loops in the dynamic control of the actin cytoskeletal network.

Citing Articles

Collective dynamics of actin and microtubule and its crosstalk mediated by FHDC1.

Tong C, Su M, Sun H, Chua X, Xiong D, Guo S Front Cell Dev Biol. 2024; 11:1261117.

PMID: 38567385 PMC: 10985548. DOI: 10.3389/fcell.2023.1261117.

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