Revolving Movement of a Dynamic Cluster of Actin Filaments During Mitosis

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2010 Oct 27

PMID 20974812

Citations 51

Authors

Masaru Mitsushima

Kazuhiro Aoki

Miki Ebisuya

Shigeru Matsumura

Takuya Yamamoto

Michiyuki Matsuda

Fumiko Toyoshima

Eisuke Nishida

Affiliations

Soon will be listed here.

Abstract

The actin cytoskeleton undergoes rapid changes in its architecture during mitosis. Here, we demonstrate novel actin assembly dynamics in M phase. An amorphous cluster of actin filaments appears during prometaphase, revolves horizontally along the cell cortex at a constant angular speed, and fuses into the contractile ring after three to four revolutions. Cdk1 activity is required for the formation of this mitotic actin cluster and its revolving movement. Rapid turnover of actin in the filaments takes place everywhere in the cluster and is also required for its cluster rotation during mitosis. Knockdown of Arp3, a component of the actin filament-nucleating Arp2/3 complex, inhibits the formation of the mitotic actin cluster without affecting other actin structures. These results identify Arp2/3 complex as a key factor in the generation of the dynamic actin cluster during mitosis.

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