Exploring the Antidiarrheal Properties of Papaya Leaf: Insights Study in Mice-Model and Analysis at M3 Muscarinic Acetylcholine Receptor Interaction

Overview

Journal Scientifica (Cairo)

Publisher Wiley

Specialty Biology

Date 2024 Jul 4

PMID 38962530

Authors

Nyi Mekar Saptarini

Faruk Jayanto Kelutur

Mary Jho-Anne Tolentino Corpuz

Affiliations

Soon will be listed here.

Abstract

Diarrhea caused by gut motility involves 5-HT serotonin receptor (5-HTR) antagonist, M3 muscarinic acetylcholine receptor (M-AchR), and H histamine receptor (HR) via their respective agonist. Papaya ( L.) leaf is an herbal medicine to treat diarrhea in Indonesia, though this has not yet been proven scientifically. This study aimed to determine the antidiarrheal properties of papaya leaf through and studies. In the mice model, papaya leaves were infused with distilled water and subjected to antidiarrheal study using castor oil-induced diarrhea. molecular docking study of nineteen secondary metabolites was carried out on the M-AchR (PDB ID: 5ZHP) using AutoDock Tools 1.5.6, while ADMET was predicted by pre-ADMET. The results showed that papaya leaf infusion caused a decrease in the total number of feces, an increase in the onset time of diarrhea, a reduction in the frequency of diarrhea, and an increase in the percentage of inhibition of diarrhea. Quercetin 3-rutinoside, a flavonoid glycoside, is potentially an antidiarrheal remedy at M-AchR. ADMET prediction showed good distribution on the target and was not toxic, but absorption needed to be considered. We concluded that the antidiarrheal activity of papaya leaf infusion was dose-dependent. Based on a molecular docking study, the flavonoid glycoside was very effective as an antidiarrheal remedy. ADMET prediction showed a specific distribution to the target and was not toxic.

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