Development of an Animal Model of Hypothyroxinemia During Pregnancy in Wistar Rats

Overview

Journal Animal Model Exp Med

Date 2024 Jul 1

PMID 38946346

Authors

Wei Wei

Aihua Liu

Min Liu

Mingfeng Li

Xinghan Wu

Chuan Qin

Zhongyan Shan

Ling Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Hypothyroxinemia is a subclinical thyroid hormone deficiency in which the mother has inadequate levels of T during pregnancy. The fetus relies entirely on the mother's T hormone level for early neurodevelopment. Isolated maternal hypothyroxinemia (IMH) in the first trimester of pregnancy can lead to lower intelligence, lower motor scores, and a higher risk of mental illness in descendants. Here, we focus on the autism-like behavior of IMH offspring.

Methods: The animals were administered 1 ppm of propylthiouracil (PTU) for 9 weeks. Then, the concentrations of T, T, and thyroid-stimulating hormone (TSH) were detected using enzyme-linked immunosorbent assay (ELISA) to verify the developed animal model of IMH. We performed four behavioral experiments, including the marble burying test, open-field test, three-chamber sociability test, and Morris water maze, to explore the autistic-like behavior of 40-day-old offspring rats.

Results: The ELISA test showed that the serum T and TSH concentrations in the model group were normal compared with the negative control group, whereas the T concentration decreased. In the behavioral experiments, the number of hidden marbles in the offspring of IMH increased significantly, the frequency of entering the central compartment decreased, and the social ratio decreased significantly.

Conclusion: The animal model of IMH was developed by the administration of 1 ppm of PTU for 9 weeks, and there were autistic-like behavior changes such as anxiety, weakened social ability, and repeated stereotyping in the IMH offspring by 40 days.

Citing Articles

Development of an animal model of hypothyroxinemia during pregnancy in Wistar rats.

Wei W, Liu A, Liu M, Li M, Wu X, Qin C Animal Model Exp Med. 2024; 7(6):926-935.

PMID: 38946346 PMC: 11680470. DOI: 10.1002/ame2.12459.

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