Elucidation of the Epitranscriptomic RNA Modification Landscape of Chikungunya Virus

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2024 Jun 27

PMID 38932237

Authors

Belinda Baquero-Perez

Enrico Bortoletto

Umberto Rosani

Anna Delgado-Tejedor

Rebeca Medina

Eva Maria Novoa

Paola Venier

Juana Diez

Affiliations

Soon will be listed here.

Abstract

The genomes of positive-sense (+) single-stranded RNA (ssRNA) viruses are believed to be subjected to a wide range of RNA modifications. In this study, we focused on the chikungunya virus (CHIKV) as a model (+) ssRNA virus to study the landscape of viral RNA modification in infected human cells. Among the 32 distinct RNA modifications analysed by mass spectrometry, inosine was found enriched in the genomic CHIKV RNA. However, orthogonal validation by Illumina RNA-seq analyses did not identify any inosine modification along the CHIKV RNA genome. Moreover, CHIKV infection did not alter the expression of ADAR1 isoforms, the enzymes that catalyse the adenosine to inosine conversion. Together, this study highlights the importance of a multidisciplinary approach to assess the presence of RNA modifications in viral RNA genomes.

Citing Articles

Bioinformatics for Inosine: Tools and Approaches to Trace This Elusive RNA Modification.

Bortoletto E, Rosani U Genes (Basel). 2024; 15(8).

PMID: 39202357 PMC: 11353476. DOI: 10.3390/genes15080996.

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