The LrhA Transcriptional Regulator Modulates Production of γ-keto--acyl Amides with Inhibitory Activity Against Mutualistic Host Nematode Egg Hatching

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2024 Jun 25

PMID 38916293

Authors

Yick Chong Lam

Randy Hamchand

Nicholas C Mucci

Sarah J Kauffman

Natavan Dudkina

Emily V Reagle

Angel M Casanova-Torres

Jessica DeCuyper

Haiwei Chen

Deguang Song

Michael G Thomas

Noah W Palm

Heidi Goodrich-Blair

Jason M Crawford

Affiliations

Soon will be listed here.

Abstract

Importance: bacteria are of interest due to their symbiotic relationship with nematodes and their ability to produce a variety of natural bioactive compounds. Despite their importance, the regulatory hierarchy connecting specific natural products and their regulators is poorly understood. In this study, comparative metabolomic profiling was utilized to identify the secondary metabolites modulated by the global regulator LrhA. This analysis led to the discovery of three metabolites, including an -acyl amide that inhibited the egg hatching rate and length of nematodes. These findings support the notion that LrhA influences the symbiosis between and through -acyl amide signaling. A deeper understanding of the regulatory hierarchy of these natural products could contribute to a better comprehension of the symbiotic relationship between and .

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