New Advances in Protein Engineering for Industrial Applications: Key Takeaways

Overview

Journal Open Life Sci

Specialty Biology

Date 2024 Jun 24

PMID 38911927

Authors

Giles Obinna Ndochinwa

Qing-Yan Wang

Nkwachukwu Oziamara Okoro

Oyetugo Chioma Amadi

Tochukwu Nwamaka Nwagu

Chukwudi Innocent Nnamchi

Anene Nwabu Moneke

Arome Solomon Odiba

Affiliations

Soon will be listed here.

Abstract

Recent advancements in protein/enzyme engineering have enabled the production of a diverse array of high-value compounds in microbial systems with the potential for industrial applications. The goal of this review is to articulate some of the most recent protein engineering advances in bacteria, yeast, and other microbial systems to produce valuable substances. These high-value substances include α-farnesene, vitamin B12, fumaric acid, linalool, glucaric acid, carminic acid, mycosporine-like amino acids, patchoulol, orcinol glucoside, d-lactic acid, keratinase, α-glucanotransferases, β-glucosidase, seleno-methylselenocysteine, fatty acids, high-efficiency β-glucosidase enzymes, cellulase, β-carotene, physcion, and glucoamylase. Additionally, recent advances in enzyme engineering for enhancing thermostability will be discussed. These findings have the potential to revolutionize various industries, including biotechnology, food, pharmaceuticals, and biofuels.

References

Rapoport A, Guzhova I, Bernetti L, Buzzini P, Kieliszek M, Kot A . Carotenoids and Some Other Pigments from Fungi and Yeasts. Metabolites. 2021; 11(2). PMC: 7915786. DOI: 10.3390/metabo11020092. View

Hartrampf N, Saebi A, Poskus M, Gates Z, Callahan A, Cowfer A . Synthesis of proteins by automated flow chemistry. Science. 2020; 368(6494):980-987. DOI: 10.1126/science.abb2491. View

Wang S, Zhan C, Nie S, Tian D, Lu J, Wen M . Enzyme and Metabolic Engineering Strategies for Biosynthesis of α-Farnesene in . J Agric Food Chem. 2023; 71(33):12452-12461. DOI: 10.1021/acs.jafc.3c03677. View

Sussman I . 65 YEARS OF THE DOUBLE HELIX: Could Watson and Crick have envisioned the true impact of their discovery?. Endocr Relat Cancer. 2018; 25(8):E9-E11. DOI: 10.1530/ERC-18-0156. View

Kieliszek M, Wasko A, Michalak K, Kot A, Piwowarek K, Winiarczyk S . Effect of selenium and methods of protein extraction on the proteomic profile of yeast. Open Life Sci. 2022; 17(1):1117-1128. PMC: 9462545. DOI: 10.1515/biol-2022-0496. View

Ennist N, Zhao Z, Stayrook S, Discher B, Dutton P, Moser C . De novo protein design of photochemical reaction centers. Nat Commun. 2022; 13(1):4937. PMC: 9399245. DOI: 10.1038/s41467-022-32710-5. View

Walker S, Yallapragada V, Tangney M . Arming Yourself for The In Silico Protein Design Revolution. Trends Biotechnol. 2020; 39(7):651-664. DOI: 10.1016/j.tibtech.2020.10.003. View

Richards F . Christian B. Anfinsen (1916-95). Nature. 1995; 376(6535):19. DOI: 10.1038/376019a0. View

Steyn A, Viljoen-Bloom M, van Zyl W . Constructing recombinant Saccharomyces cerevisiae strains for malic-to-fumaric acid conversion. FEMS Microbiol Lett. 2023; 370. PMC: 10086307. DOI: 10.1093/femsle/fnad003. View

10.

Dryden D, Thomson A, White J . How much of protein sequence space has been explored by life on Earth?. J R Soc Interface. 2008; 5(25):953-6. PMC: 2459213. DOI: 10.1098/rsif.2008.0085. View

11.

Zhang H, Zhao A, Qu L, Xiong W, Alam M, Miao J . Engineering an efficient whole-cell catalyst for d-allulose production from glycerol. Biotechnol J. 2023; 18(7):e2200600. DOI: 10.1002/biot.202200600. View

12.

Yan Y, Bai Y, Zheng X, Cai Y . Production of hydroxytyrosol through whole-cell bioconversion from L-DOPA using engineered Escherichia coli. Enzyme Microb Technol. 2023; 169:110280. DOI: 10.1016/j.enzmictec.2023.110280. View

13.

Ormandy E, Dale J, Griffin G . Genetic engineering of animals: ethical issues, including welfare concerns. Can Vet J. 2011; 52(5):544-50. PMC: 3078015. View

14.

Entzeroth M, Flotow H, Condron P . Overview of high-throughput screening. Curr Protoc Pharmacol. 2012; Chapter 9:Unit 9.4. DOI: 10.1002/0471141755.ph0904s44. View

15.

Zhou P, Zhou X, Yuan D, Fang X, Pang X, Yuan K . Combining Protein and Organelle Engineering for Linalool Overproduction in . J Agric Food Chem. 2023; 71(26):10133-10143. DOI: 10.1021/acs.jafc.2c08416. View

16.

Lehner S, Boles E . Development of vitamin B12 dependency in Saccharomyces cerevisiae. FEMS Yeast Res. 2023; 23. DOI: 10.1093/femsyr/foad020. View

17.

JOHNSON I . Human insulin from recombinant DNA technology. Science. 1983; 219(4585):632-7. DOI: 10.1126/science.6337396. View

18.

Zhang Y, An N, Zhao Y, Li X, Shen X, Wang J . Efficient biosynthesis of α-aminoadipic acid via lysine catabolism in Escherichia coli. Biotechnol Bioeng. 2022; 120(1):312-317. DOI: 10.1002/bit.28256. View

19.

Kieliszek M, Blazejak S, Bzducha-Wrobel A, Kot A . Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells. Biol Trace Elem Res. 2018; 187(1):316-327. PMC: 6315055. DOI: 10.1007/s12011-018-1342-x. View

20.

Dong S, Sun J, Mao Z, Wang L, Lu Y, Li J . A guideline for homology modeling of the proteins from newly discovered betacoronavirus, 2019 novel coronavirus (2019-nCoV). J Med Virol. 2020; 92(9):1542-1548. PMC: 7228330. DOI: 10.1002/jmv.25768. View