Improved Biocompatibility and Osseointegration of Nanostructured Calcium-Incorporated Titanium Implant Surface Treatment (XPEED)

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2024 Jun 19

PMID 38893971

Authors

Kyung Ran Yang

Min-Ho Hong

Affiliations

Soon will be listed here.

Abstract

Surface treatment of implants facilitates osseointegration, with nanostructured surfaces exhibiting accelerated peri-implant bone regeneration. This study compared bone-to-implant contact (BIC) in implants with hydroxyapatite (HA), sand-blasted and acid-etched (SLA), and SLA with calcium (Ca)-coated (XPEED) surfaces. Seventy-five disk-shaped grade 4 Ti specimens divided into three groups were prepared, with 16 implants per group tested in New Zealand white rabbits. Surface characterization was performed using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), digital microscopy, and a contact angle analyzer. Cell viability, proliferation, and adhesion were assessed using MC3T3-E1 cells. Apatite formation was evaluated using modified simulated body fluid (m-SBF) incubation. After 4 weeks of healing, the outcomes reviewed were BIC, bone area (BA), removal torque tests, and histomorphometric evaluation. A microstructure analysis revealed irregular pores across all groups, with the XPEED group exhibiting a nanostructured Ca-coated surface. Surface characterization showed a crystalline CaTiO layer on XPEED surfaces, with evenly distributed Ca penetrating the implants. All surfaces provided excellent environments for cell growth. The XPEED and SLA groups showed significantly higher cell density and viability with superior osseointegration than HA ( < 0.05); XPEED exhibited the highest absorbance values. Thus, XPEED surface treatment improved implant performance, biocompatibility, stability, and osseointegration.

References

Nayab S, Jones F, Olsen I . Effects of calcium ion implantation on human bone cell interaction with titanium. Biomaterials. 2005; 26(23):4717-27. DOI: 10.1016/j.biomaterials.2004.11.044. View

Smeets R, Stadlinger B, Schwarz F, Beck-Broichsitter B, Jung O, Precht C . Impact of Dental Implant Surface Modifications on Osseointegration. Biomed Res Int. 2016; 2016:6285620. PMC: 4958483. DOI: 10.1155/2016/6285620. View

Brett P, Harle J, Salih V, Mihoc R, Olsen I, Jones F . Roughness response genes in osteoblasts. Bone. 2004; 35(1):124-33. DOI: 10.1016/j.bone.2004.03.009. View

Bernhardt R, Kuhlisch E, Schulz M, Eckelt U, Stadlinger B . Comparison of bone-implant contact and bone-implant volume between 2D-histological sections and 3D-SRµCT slices. Eur Cell Mater. 2012; 23:237-47. DOI: 10.22203/ecm.v023a18. View

Barrere F, van der Valk C, Meijer G, Dalmeijer R, de Groot K, Layrolle P . Osteointegration of biomimetic apatite coating applied onto dense and porous metal implants in femurs of goats. J Biomed Mater Res B Appl Biomater. 2003; 67(1):655-65. DOI: 10.1002/jbm.b.10057. View

Suh J, Jeung O, Choi B, Park J . Effects of a novel calcium titanate coating on the osseointegration of blasted endosseous implants in rabbit tibiae. Clin Oral Implants Res. 2007; 18(3):362-9. DOI: 10.1111/j.1600-0501.2006.01323.x. View

Buser D, Broggini N, Wieland M, Schenk R, Denzer A, Cochran D . Enhanced bone apposition to a chemically modified SLA titanium surface. J Dent Res. 2004; 83(7):529-33. DOI: 10.1177/154405910408300704. View

Albrektsson T, Branemark P, Hansson H, Lindstrom J . Osseointegrated titanium implants. Requirements for ensuring a long-lasting, direct bone-to-implant anchorage in man. Acta Orthop Scand. 1981; 52(2):155-70. DOI: 10.3109/17453678108991776. View

McCullough J, Klokkevold P . The effect of implant macro-thread design on implant stability in the early post-operative period: a randomized, controlled pilot study. Clin Oral Implants Res. 2016; 28(10):1218-1226. DOI: 10.1111/clr.12945. View

10.

Shibli J, Grassi S, Figueiredo L, Feres M, Marcantonio Jr E, Iezzi G . Influence of implant surface topography on early osseointegration: a histological study in human jaws. J Biomed Mater Res B Appl Biomater. 2006; 80(2):377-85. DOI: 10.1002/jbm.b.30608. View

11.

Park J, Kim H, Kim Y, An C, Hanawa T . Enhanced osteoconductivity of micro-structured titanium implants (XiVE S CELLplus) by addition of surface calcium chemistry: a histomorphometric study in the rabbit femur. Clin Oral Implants Res. 2009; 20(7):684-90. DOI: 10.1111/j.1600-0501.2009.01714.x. View

12.

Le Guehennec L, Lopez-Heredia M, Enkel B, Weiss P, Amouriq Y, Layrolle P . Osteoblastic cell behaviour on different titanium implant surfaces. Acta Biomater. 2008; 4(3):535-43. DOI: 10.1016/j.actbio.2007.12.002. View

13.

Borghi F, Bean P, Evans M, van der Laan T, Kumar S, Ostrikov K . Nanostructured Graphene Surfaces Promote Different Stages of Bone Cell Differentiation. Nanomicro Lett. 2018; 10(3):47. PMC: 6199093. DOI: 10.1007/s40820-018-0198-0. View

14.

Zhang J, Xie Y, Zuo J, Li J, Wei Q, Yu Z . Cell responses to titanium treated by a sandblast-free method for implant applications. Mater Sci Eng C Mater Biol Appl. 2017; 78:1187-1194. DOI: 10.1016/j.msec.2017.04.119. View

15.

Ryoo S, Kim Y, Kim M, Min D . Behaviors of NIH-3T3 fibroblasts on graphene/carbon nanotubes: proliferation, focal adhesion, and gene transfection studies. ACS Nano. 2010; 4(11):6587-98. DOI: 10.1021/nn1018279. View

16.

Serro A, Saramago B . Influence of sterilization on the mineralization of titanium implants induced by incubation in various biological model fluids. Biomaterials. 2003; 24(26):4749-60. DOI: 10.1016/s0142-9612(03)00372-7. View

17.

Hanisch O, Cortella C, Boskovic M, James R, Slots J, Wikesjo U . Experimental peri-implant tissue breakdown around hydroxyapatite-coated implants. J Periodontol. 1997; 68(1):59-66. DOI: 10.1902/jop.1997.68.1.59. View

18.

Junker R, Dimakis A, Thoneick M, Jansen J . Effects of implant surface coatings and composition on bone integration: a systematic review. Clin Oral Implants Res. 2009; 20 Suppl 4:185-206. DOI: 10.1111/j.1600-0501.2009.01777.x. View

19.

Park Y, Yi K, Lee I, Jung Y . Correlation between microtomography and histomorphometry for assessment of implant osseointegration. Clin Oral Implants Res. 2005; 16(2):156-60. DOI: 10.1111/j.1600-0501.2004.01083.x. View

20.

Bowers K, Keller J, Randolph B, Wick D, Michaels C . Optimization of surface micromorphology for enhanced osteoblast responses in vitro. Int J Oral Maxillofac Implants. 1992; 7(3):302-10. View