Characterizations on a GRAS Electrospun Lipid-Polymer Composite Loaded with Tetrahydrocurcumin

Overview

Journal Foods

Specialty Biotechnology

Date 2024 Jun 19

PMID 38890901

Authors

Zhenyu Lin

Jun Li

Qingrong Huang

Affiliations

Soon will be listed here.

Abstract

Electrospun/sprayed fiber films and nanoparticles were broadly studied as encapsulation techniques for bioactive compounds. Nevertheless, many of them involved using non-volatile toxic solvents or non-biodegradable polymers that were not suitable for oral consumption, thus rather limiting their application. In this research, a novel electrospun lipid-polymer composite (ELPC) was fabricated with whole generally recognized as safe (GRAS) materials including gelatin, medium chain triglyceride (MCT) and lecithin. A water-insoluble bioactive compound, tetrahydrocurcumin (TC), was encapsulated in the ELPC to enhance its delivery. Confocal laser scanning microscopy (CLSM) was utilized to examine the morphology of this ELPC and found that it was in a status between electrospun fibers and electrosprayed particles. It was able to form self-assembled emulsions (droplets visualized by CLSM) to deliver active compounds. In addition, this gelatin-based ELPC self-assembled emulsion was able to form a special emulsion gel. CLSM observation of this gel displayed that the lipophilic contents of the ELPC were encapsulated within the cluster of the hydrophilic gelatin gel network. The FTIR spectrum of the TC-loaded ELPC did not show the fingerprint pattern of crystalline TC, while it displayed the aliphatic hydrocarbon stretches from MCT and lecithin. The dissolution experiment demonstrated a relatively linear release profile of TC from the ELPC. The lipid digestion assay displayed a rapid digestion of triglycerides in the first 3-6 min, with a high extent of lipolysis. A Caco-2 intestinal monolayer transport study was performed. The ELPC delivered more TC in the upward direction than downwards. MTT study results did not report cytotoxicity for both pure TC and the ELPC-encapsulated TC under 15 μg/mL. Caco-2 cellular uptake was visualized by CLSM and semi-quantified to estimate the accumulation rate of TC in the cells over time.

References

Cheng C, Wang R, Ma J, Zhang Y, Jing Q, Lu W . Examining the wound healing potential of curcumin-infused electrospun nanofibers from polyglutamic acid and gum arabic. Int J Biol Macromol. 2024; 267(Pt 1):131237. DOI: 10.1016/j.ijbiomac.2024.131237. View

Vecchione R, Quagliariello V, Calabria D, Calcagno V, De Luca E, Iaffaioli R . Curcumin bioavailability from oil in water nano-emulsions: In vitro and in vivo study on the dimensional, compositional and interactional dependence. J Control Release. 2016; 233:88-100. DOI: 10.1016/j.jconrel.2016.05.004. View

Fernandez-Garcia E, Carvajal-Lerida I, Perez-Galvez A . In vitro bioaccessibility assessment as a prediction tool of nutritional efficiency. Nutr Res. 2009; 29(11):751-60. DOI: 10.1016/j.nutres.2009.09.016. View

Pari L, Amali D . Protective role of tetrahydrocurcumin (THC) an active principle of turmeric on chloroquine induced hepatotoxicity in rats. J Pharm Pharm Sci. 2005; 8(1):115-23. View

Sharma J, Bhatt S, Tiwari A, Tiwari V, Kumar M, Verma R . Statistical optimization of tetrahydrocurcumin loaded solid lipid nanoparticles using Box Behnken design in the management of streptozotocin-induced diabetes mellitus. Saudi Pharm J. 2023; 31(9):101727. PMC: 10448172. DOI: 10.1016/j.jsps.2023.101727. View

Carbonell-Capella J, Buniowska M, Barba F, Esteve M, Frigola A . Analytical Methods for Determining Bioavailability and Bioaccessibility of Bioactive Compounds from Fruits and Vegetables: A Review. Compr Rev Food Sci Food Saf. 2021; 13(2):155-171. DOI: 10.1111/1541-4337.12049. View

Park S, Lee L, Seo J, Kang S . Curcumin and tetrahydrocurcumin both prevent osteoarthritis symptoms and decrease the expressions of pro-inflammatory cytokines in estrogen-deficient rats. Genes Nutr. 2016; 11:2. PMC: 4959551. DOI: 10.1186/s12263-016-0520-4. View

Yu H, Huang Q . Investigation of the cytotoxicity of food-grade nanoemulsions in Caco-2 cell monolayers and HepG2 cells. Food Chem. 2013; 141(1):29-33. DOI: 10.1016/j.foodchem.2013.03.009. View

Ting Y, Li C, Pan M, Ho C, Huang Q . Effect of a labile methyl donor on the transformation of 5-demethyltangeretin and the related implication on bioactivity. J Agric Food Chem. 2013; 61(34):8090-7. DOI: 10.1021/jf400562p. View

10.

Lai C, Ho C, Pan M . The Cancer Chemopreventive and Therapeutic Potential of Tetrahydrocurcumin. Biomolecules. 2020; 10(6). PMC: 7356876. DOI: 10.3390/biom10060831. View

11.

Yu H, Huang Q . Improving the oral bioavailability of curcumin using novel organogel-based nanoemulsions. J Agric Food Chem. 2012; 60(21):5373-9. DOI: 10.1021/jf300609p. View

12.

Sandur S, Pandey M, Sung B, Ahn K, Murakami A, Sethi G . Curcumin, demethoxycurcumin, bisdemethoxycurcumin, tetrahydrocurcumin and turmerones differentially regulate anti-inflammatory and anti-proliferative responses through a ROS-independent mechanism. Carcinogenesis. 2007; 28(8):1765-73. DOI: 10.1093/carcin/bgm123. View

13.

Pari L, Murugan P . Effect of tetrahydrocurcumin on blood glucose, plasma insulin and hepatic key enzymes in streptozotocin induced diabetic rats. J Basic Clin Physiol Pharmacol. 2006; 16(4):257-74. DOI: 10.1515/jbcpp.2005.16.4.257. View

14.

Liu D, Cheng B, Li D, Li J, Wu Q, Pan H . Investigations on the interactions between curcumin loaded vitamin E TPGS coated nanodiamond and Caco-2 cell monolayer. Int J Pharm. 2018; 551(1-2):177-183. DOI: 10.1016/j.ijpharm.2018.09.030. View

15.

Turck D, Bohn T, Castenmiller J, de Henauw S, Hirsch-Ernst K, Maciuk A . Safety of tetrahydrocurcuminoids from turmeric ( L.) as a novel food pursuant to Regulation (EU) 2015/2283. EFSA J. 2022; 19(12):e06936. PMC: 8693248. DOI: 10.2903/j.efsa.2021.6936. View

16.

Li X, Li Y, Yu D, Liao Y, Wang X . Fast disintegrating quercetin-loaded drug delivery systems fabricated using coaxial electrospinning. Int J Mol Sci. 2013; 14(11):21647-59. PMC: 3856026. DOI: 10.3390/ijms141121647. View

17.

Loron A, Navikaite-Snipaitiene V, Rosliuk D, Rutkaite R, Gardrat C, Coma V . Polysaccharide Matrices for the Encapsulation of Tetrahydrocurcumin-Potential Application as Biopesticide against . Molecules. 2021; 26(13). PMC: 8270288. DOI: 10.3390/molecules26133873. View

18.

Fanca-Berthon P, Tenon M, Bouter-Banon S, Manfre A, Maudet C, Dion A . Pharmacokinetics of a Single Dose of Turmeric Curcuminoids Depends on Formulation: Results of a Human Crossover Study. J Nutr. 2021; 151(7):1802-1816. PMC: 8245892. DOI: 10.1093/jn/nxab087. View

19.

Li M, Cui J, Ngadi M, Ma Y . Absorption mechanism of whey-protein-delivered curcumin using Caco-2 cell monolayers. Food Chem. 2015; 180:48-54. DOI: 10.1016/j.foodchem.2015.01.132. View

20.

Wang Y, Pan M, Cheng A, Lin L, Ho Y, Hsieh C . Stability of curcumin in buffer solutions and characterization of its degradation products. J Pharm Biomed Anal. 1997; 15(12):1867-76. DOI: 10.1016/s0731-7085(96)02024-9. View