Hyperbaric Oxygen Enhances Tumor Penetration and Accumulation of Engineered Bacteria for Synergistic Photothermal Immunotherapy

Overview

Journal Nat Commun

Specialty Biology

Date 2024 Jun 17

PMID 38886343

Authors

Ke-Fei Xu

Shun-Yu Wu

Zihao Wang

Yuxin Guo

Ya-Xuan Zhu

Chengcheng Li

Bai-Hui Shan

Xinping Zhang

Xiaoyang Liu

Fu-Gen Wu

Affiliations

Soon will be listed here.

Abstract

Bacteria-mediated cancer therapeutic strategies have attracted increasing interest due to their intrinsic tumor tropism. However, bacteria-based drugs face several challenges including the large size of bacteria and dense extracellular matrix, limiting their intratumoral delivery efficiency. In this study, we find that hyperbaric oxygen (HBO), a noninvasive therapeutic method, can effectively deplete the dense extracellular matrix and thus enhance the bacterial accumulation within tumors. Inspired by this finding, we modify Escherichia coli Nissle 1917 (EcN) with cypate molecules to yield EcN-cypate for photothermal therapy, which can subsequently induce immunogenic cell death (ICD). Importantly, HBO treatment significantly increases the intratumoral accumulation of EcN-cypate and facilitates the intratumoral infiltration of immune cells to realize desirable tumor eradication through photothermal therapy and ICD-induced immunotherapy. Our work provides a facile and noninvasive strategy to enhance the intratumoral delivery efficiency of natural/engineered bacteria, and may promote the clinical translation of bacteria-mediated synergistic cancer therapy.

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