Determination of Differential Alternative Splicing Under Stress Conditions

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Jun 13

PMID 38869788

Authors

Paola Punzo

Riccardo Suede Cigliano

Riccardo Aversano

Stefania Grillo

Giorgia Batelli

Affiliations

Soon will be listed here.

Abstract

Alternative splicing (AS) is an important mechanism contributing to stress-induced regulation of gene expression and proteome diversity. Massive sequencing technologies allow the identification of transcripts generated via stress-responsive AS, potentially important for adaptation to stress conditions. Several bioinformatics tools have been developed to identify differentially expressed alternative splicing events/transcripts from RNA-sequencing results. This chapter describes a detailed protocol for differential alternative splicing analysis using the rMATS tool. In addition, we provide guidelines for validation of the detected splice variants by qRT-PCR based on the obtained output files.

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