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The Lack of Synapsin Alters Presynaptic Plasticity at Hippocampal Mossy Fibers in Male Mice

Overview
Journal eNeuro
Specialty Neurology
Date 2024 Jun 12
PMID 38866497
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Abstract

Synapsins are highly abundant presynaptic proteins that play a crucial role in neurotransmission and plasticity via the clustering of synaptic vesicles. The synapsin III isoform is usually downregulated after development, but in hippocampal mossy fiber boutons, it persists in adulthood. Mossy fiber boutons express presynaptic forms of short- and long-term plasticity, which are thought to underlie different forms of learning. Previous research on synapsins at this synapse focused on synapsin isoforms I and II. Thus, a complete picture regarding the role of synapsins in mossy fiber plasticity is still missing. Here, we investigated presynaptic plasticity at hippocampal mossy fiber boutons by combining electrophysiological field recordings and transmission electron microscopy in a mouse model lacking all synapsin isoforms. We found decreased short-term plasticity, i.e., decreased facilitation and post-tetanic potentiation, but increased long-term potentiation in male synapsin triple knock-out (KO) mice. At the ultrastructural level, we observed more dispersed vesicles and a higher density of active zones in mossy fiber boutons from KO animals. Our results indicate that all synapsin isoforms are required for fine regulation of short- and long-term presynaptic plasticity at the mossy fiber synapse.

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References
1.
Nadler J . The recurrent mossy fiber pathway of the epileptic brain. Neurochem Res. 2003; 28(11):1649-58. DOI: 10.1023/a:1026004904199. View

2.
Takei Y, Harada A, Takeda S, Kobayashi K, Terada S, Noda T . Synapsin I deficiency results in the structural change in the presynaptic terminals in the murine nervous system. J Cell Biol. 1995; 131(6 Pt 2):1789-800. PMC: 2120677. DOI: 10.1083/jcb.131.6.1789. View

3.
Cheng Q, Song S, Augustine G . Molecular Mechanisms of Short-Term Plasticity: Role of Synapsin Phosphorylation in Augmentation and Potentiation of Spontaneous Glutamate Release. Front Synaptic Neurosci. 2018; 10:33. PMC: 6218601. DOI: 10.3389/fnsyn.2018.00033. View

4.
Harte-Hargrove L, Maclusky N, Scharfman H . Brain-derived neurotrophic factor-estrogen interactions in the hippocampal mossy fiber pathway: implications for normal brain function and disease. Neuroscience. 2013; 239:46-66. PMC: 3628287. DOI: 10.1016/j.neuroscience.2012.12.029. View

5.
Torborg C, Nakashiba T, Tonegawa S, McBain C . Control of CA3 output by feedforward inhibition despite developmental changes in the excitation-inhibition balance. J Neurosci. 2010; 30(46):15628-37. PMC: 3023412. DOI: 10.1523/JNEUROSCI.3099-10.2010. View