Fluorescent Nitrogen-doped Carbon Dots-based Turn-off Sensor for Bilirubin

Overview

Journal J Fluoresc

Specialties Biophysics
Chemistry

Date 2024 Jun 12

PMID 38865062

Authors

Aswathy A O

B A Akhila

Sony George

Affiliations

Soon will be listed here.

Abstract

Bilirubin (BR), a heme protein produced from breakdown of haemoglobin is present in aged red blood cells; whose abnormal concentration is associated with diseases like hyperbilirubinemia, coronary disease, iron deficiency, and so on. Herein, we have synthesized a selective, sensitive, and low-cost sensing platform using fluorescent nitrogen doped carbon dots (NCDs), prepared from precursors; citric acid and urea via a simple microwave-assisted method. The emission at 444 nm on excitation with 360 nm was well quenched in presence of BR suggesting a direct turn-off detection for BR. Characterization of developed probe was done by UV-Visible absorption studies, photoluminescence studies, SEM, TEM, ATR-FTIR, XPS, and DLS analysis. BR was detected with a Limit of Detection (LoD) and Limit of Quantification (LoQ) of 0.32 µM and 1.08 µM respectively. NCDs exhibited excellent selectivity and sensitivity towards BR in the presence of co-existing biomolecules and ions. Practical feasibility was checked by paper-strip-based sensing of BR and spiked real human samples were used for conducting real sample analysis.

Citing Articles

Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin.

Lakshmi Devi A, Sreelakshmi M, Suneesh P, Satheesh Babu T Sci Rep. 2025; 15(1):1262.

PMID: 39779895 PMC: 11711382. DOI: 10.1038/s41598-025-85246-1.

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