Dynamic Label-free Analysis of SARS-CoV-2 Infection Reveals Virus-induced Subcellular Remodeling

Overview

Journal Nat Commun

Specialty Biology

Date 2024 Jun 11

PMID 38862527

Authors

Nell Saunders

Blandine Monel

Nadege Cayet

Lorenzo Archetti

Hugo Moreno

Alexandre Jeanne

Agathe Marguier

Julian Buchrieser

Timothy Wai

Olivier Schwartz

Mathieu Frechin

Affiliations

Soon will be listed here.

Abstract

Assessing the impact of SARS-CoV-2 on organelle dynamics allows a better understanding of the mechanisms of viral replication. We combine label-free holotomographic microscopy with Artificial Intelligence to visualize and quantify the subcellular changes triggered by SARS-CoV-2 infection. We study the dynamics of shape, position and dry mass of nucleoli, nuclei, lipid droplets and mitochondria within hundreds of single cells from early infection to syncytia formation and death. SARS-CoV-2 infection enlarges nucleoli, perturbs lipid droplets, changes mitochondrial shape and dry mass, and separates lipid droplets from mitochondria. We then used Bayesian network modeling on organelle dry mass states to define organelle cross-regulation networks and report modifications of organelle cross-regulation that are triggered by infection and syncytia formation. Our work highlights the subcellular remodeling induced by SARS-CoV-2 infection and provides an Artificial Intelligence-enhanced, label-free methodology to study in real-time the dynamics of cell populations and their content.

Citing Articles

Mantis: High-throughput 4D imaging and analysis of the molecular and physical architecture of cells.

Ivanov I, Hirata-Miyasaki E, Chandler T, Cheloor-Kovilakam R, Liu Z, Pradeep S PNAS Nexus. 2024; 3(9):pgae323.

PMID: 39282007 PMC: 11393572. DOI: 10.1093/pnasnexus/pgae323.

Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells.

Ivanov I, Hirata-Miyasaki E, Chandler T, Cheloor-Kovilakam R, Liu Z, Pradeep S bioRxiv. 2024; .

PMID: 38187521 PMC: 10769231. DOI: 10.1101/2023.12.19.572435.

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