Integrated OMICs Approach Reveals Energy Metabolism Pathway is Vital for Pullorum Survival Within the Egg White

Overview

Journal mSphere

Publisher American Society for Microbiology

Specialties Microbiology
Parasitology

Date 2024 Jun 11

PMID 38860771

Authors

Xiamei Kang

Hongli An

Baikui Wang

Linlin Huang

Chenghu Huang

Yingying Huang

Zining Wang

Fang He

Yan Li

Min Yue

Affiliations

Soon will be listed here.

Abstract

Importance: Pullorum disease, causing serious embryo death and chick mortality, results in substantial economic losses worldwide due to transovarial transmission. Egg-borne outbreaks are frequently reported in many countries. The present study has filled the knowledge gap regarding how the specific chicken-adapted pathogen Pullorum behaves within the challenging environment of egg white. The deletion of the fimbrial system can increase survival in the albumen, possibly by reprogramming metabolism-related gene products, which reveals a new adaptive strategy of pathogens. Moreover, the comparison, including previous research on Enteritidis, capable of vertical transmission, aims to provide diversified data sets in the field and further help to implement reasonable and effective measures to improve both food safety and animal health.

Citing Articles

Avian-specific transition to endemicity is accompanied by localized resistome and mobilome interaction.

Jia C, Huang C, Zhou H, Zhou X, Wang Z, Siddique A Elife. 2025; 13.

PMID: 40035424 PMC: 11879110. DOI: 10.7554/eLife.101241.

Genomic and resistome analysis of isolates from retail markets in Yichun city, China.

Jiang X, Siddique A, Chen L, Zhu L, Zhou H, Na L One Health. 2025; 20:100967.

PMID: 39906162 PMC: 11791297. DOI: 10.1016/j.onehlt.2025.100967.

Developing a novel TaqMan qPCR assay for optimizing Pullorum detection in chickens.

Wang H, Kang X, Yu L, Wang H, Muller A, Kehrenberg C Vet Q. 2025; 45(1):1-13.

PMID: 39882692 PMC: 11784030. DOI: 10.1080/01652176.2025.2454473.

Sodium dodecyl sulfate-coated silver nanoparticles accelerate antimicrobial potentials by targeting amphiphilic membranes.

Jin X, Peng N, Cui A, Liu Y, Peng X, Huang L mLife. 2025; 3(4):551-564.

PMID: 39744089 PMC: 11686091. DOI: 10.1002/mlf2.12143.

Genomic and virulent characterization of a duck-associated Salmonella serovar Potsdam from China.

An H, Kang X, Huang C, Jia C, Chen J, Huang Y Poult Sci. 2024; 104(1):104646.

PMID: 39693960 PMC: 11719287. DOI: 10.1016/j.psj.2024.104646.

References

Zhou X, Kang X, Zhou K, Yue M . A global dataset for prevalence of Salmonella Gallinarum between 1945 and 2021. Sci Data. 2022; 9(1):495. PMC: 9376096. DOI: 10.1038/s41597-022-01605-x. View

Chen J, Zhou X, Tang Y, Jiang Z, Kang X, Wang J . Characterization of two-component system CitB family in Salmonella enterica serovar Gallinarum biovar Gallinarum. Vet Microbiol. 2023; 278:109659. DOI: 10.1016/j.vetmic.2023.109659. View

Ray S, Kassan A, Busija A, Rangamani P, Patel H . The plasma membrane as a capacitor for energy and metabolism. Am J Physiol Cell Physiol. 2016; 310(3):C181-92. PMC: 4888523. DOI: 10.1152/ajpcell.00087.2015. View

Goff J, Yee N . Tellurate enters Escherichia coli K-12 cells via the SulT-type sulfate transporter CysPUWA. FEMS Microbiol Lett. 2017; 364(24). DOI: 10.1093/femsle/fnx241. View

Lamichhane-Khadka R, Benoit S, Miller-Parks E, Maier R . Host hydrogen rather than that produced by the pathogen is important for Salmonella enterica serovar Typhimurium virulence. Infect Immun. 2014; 83(1):311-6. PMC: 4288883. DOI: 10.1128/IAI.02611-14. View

Lamichhane-Khadka R, Benoit S, Maier S, Maier R . A link between gut community metabolism and pathogenesis: molecular hydrogen-stimulated glucarate catabolism aids Salmonella virulence. Open Biol. 2013; 3(12):130146. PMC: 3877842. DOI: 10.1098/rsob.130146. View

Gantois I, Ducatelle R, Pasmans F, Haesebrouck F, Gast R, Humphrey T . Mechanisms of egg contamination by Salmonella Enteritidis. FEMS Microbiol Rev. 2009; 33(4):718-38. DOI: 10.1111/j.1574-6976.2008.00161.x. View

Langridge G, Fookes M, Connor T, Feltwell T, Feasey N, Parsons B . Patterns of genome evolution that have accompanied host adaptation in Salmonella. Proc Natl Acad Sci U S A. 2014; 112(3):863-8. PMC: 4311825. DOI: 10.1073/pnas.1416707112. View

McClelland M, Sanderson K, Spieth J, Clifton S, Latreille P, Courtney L . Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 2001; 413(6858):852-6. DOI: 10.1038/35101614. View

10.

Raspoet R, Appia-Ayme C, Shearer N, Martel A, Pasmans F, Haesebrouck F . Microarray-based detection of Salmonella enterica serovar Enteritidis genes involved in chicken reproductive tract colonization. Appl Environ Microbiol. 2014; 80(24):7710-6. PMC: 4249237. DOI: 10.1128/AEM.02867-14. View

11.

Baron F, Cochet M, Alabdeh M, Guerin-Dubiard C, Gautier M, Nau F . Egg-White Proteins Have a Minor Impact on the Bactericidal Action of Egg White Toward Enteritidis at 45°C. Front Microbiol. 2020; 11:584986. PMC: 7578404. DOI: 10.3389/fmicb.2020.584986. View

12.

Beney L, Gervais P . Influence of the fluidity of the membrane on the response of microorganisms to environmental stresses. Appl Microbiol Biotechnol. 2001; 57(1-2):34-42. DOI: 10.1007/s002530100754. View

13.

Berchieri Jr A, Murphy C, Marston K, Barrow P . Observations on the persistence and vertical transmission of Salmonella enterica serovars Pullorum and Gallinarum in chickens: effect of bacterial and host genetic background. Avian Pathol. 2009; 30(3):221-31. DOI: 10.1080/03079450120054631. View

14.

Li Y, Ed-Dra A, Tang B, Kang X, Muller A, Kehrenberg C . Higher tolerance of predominant Salmonella serovars circulating in the antibiotic-free feed farms to environmental stresses. J Hazard Mater. 2022; 438:129476. DOI: 10.1016/j.jhazmat.2022.129476. View

15.

Zhou X, Kang X, Chen J, Song Y, Jia C, Teng L . Genome degradation promotes pathoadaptation by remodeling fimbriae-mediated proinflammatory response. Natl Sci Rev. 2023; 10(10):nwad228. PMC: 10642762. DOI: 10.1093/nsr/nwad228. View

16.

De Buck J, Van Immerseel F, Haesebrouck F, Ducatelle R . Effect of type 1 fimbriae of Salmonella enterica serotype Enteritidis on bacteraemia and reproductive tract infection in laying hens. Avian Pathol. 2004; 33(3):314-20. DOI: 10.1080/0307945042000220561. View

17.

Baron F, Nau F, Guerin-Dubiard C, Bonnassie S, Gautier M, Andrews S . Egg white versus Salmonella Enteritidis! A harsh medium meets a resilient pathogen. Food Microbiol. 2015; 53(Pt B):82-93. DOI: 10.1016/j.fm.2015.09.009. View

18.

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View

19.

Wei Y, Miller C . Characterization of a group of anaerobically induced, fnr-dependent genes of Salmonella typhimurium. J Bacteriol. 1999; 181(19):6092-7. PMC: 103637. DOI: 10.1128/JB.181.19.6092-6097.1999. View

20.

Kang X, Zhou X, Tang Y, Jiang Z, Chen J, Mohsin M . Characterization of Two-Component System CitB Family in Pullorum. Int J Mol Sci. 2022; 23(17). PMC: 9456408. DOI: 10.3390/ijms231710201. View