Construction of Telocentric Chromosomes in Saccharomyces Cerevisiae

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Apr 1

PMID 3885221

Citations 25

Authors

R T Surosky

B K Tye

Affiliations

Soon will be listed here.

Abstract

We describe a simple method for the construction of large chromosomal deletions in yeast. Diploid yeast cells were transformed with DNA fragments that replace large regions of the chromosomes by homologous recombination. Using this method, we have constructed a telocentric chromosome III in which approximately equal to 100 kilobases (kb) of DNA has been removed from the left arm of the chromosome, so that the centromere is 12 kb from the left telomere. This telocentric chromosome is mitotically stable. Its rate of loss in a diploid strain is 2.5-7.4 X 10(-4) per cell division compared to a rate of loss of 0.36-1.8 X 10(-4) per cell division for a normal chromosome III. It also segregates 2+:2- with fidelity during meiosis. The construction of systematic deletions in a chromosome should be useful in determining the essential features for proper chromosomal segregation and replication.

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References

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Clarke L, Carbon J . Isolation of a yeast centromere and construction of functional small circular chromosomes. Nature. 1980; 287(5782):504-9. DOI: 10.1038/287504a0. View

Sora S, Lucchini G, Magni G . Meiotic Diploid Progeny and Meiotic Nondisjunction in SACCHAROMYCES CEREVISIAE. Genetics. 1982; 101(1):17-33. PMC: 1201848. DOI: 10.1093/genetics/101.1.17. View

Kingsman A, Gimlich R, Clarke L, Chinault A, Carbon J . Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae. J Mol Biol. 1981; 145(4):619-32. DOI: 10.1016/0022-2836(81)90306-5. View

Morrison D . Transformation in Escherichia coli: cryogenic preservation of competent cells. J Bacteriol. 1977; 132(1):349-51. PMC: 221863. DOI: 10.1128/jb.132.1.349-351.1977. View

Holmes D, Quigley M . A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981; 114(1):193-7. DOI: 10.1016/0003-2697(81)90473-5. View

Cryer D, Eccleshall R, MARMUR J . Isolation of yeast DNA. Methods Cell Biol. 1975; 12:39-44. DOI: 10.1016/s0091-679x(08)60950-4. View

Shortle D, Haber J, Botstein D . Lethal disruption of the yeast actin gene by integrative DNA transformation. Science. 1982; 217(4557):371-3. DOI: 10.1126/science.7046050. View

Chan C, Tye B . A family of Saccharomyces cerevisiae repetitive autonomously replicating sequences that have very similar genomic environments. J Mol Biol. 1983; 168(3):505-23. DOI: 10.1016/s0022-2836(83)80299-x. View

10.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

11.

Rothstein R . One-step gene disruption in yeast. Methods Enzymol. 1983; 101:202-11. DOI: 10.1016/0076-6879(83)01015-0. View

12.

Rabkin S, Friesen J, FERRIS J, Fung H . A model of cardiac arrhythmias and sudden death: cantharidin-induced toxic cardiomyopathy. J Pharmacol Exp Ther. 1979; 210(1):43-50. View

13.

Hinnen A, Hicks J, Fink G . Transformation of yeast. Proc Natl Acad Sci U S A. 1978; 75(4):1929-33. PMC: 392455. DOI: 10.1073/pnas.75.4.1929. View

14.

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

15.

Luria S, DELBRUCK M . Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943; 28(6):491-511. PMC: 1209226. DOI: 10.1093/genetics/28.6.491. View

16.

Dunn B, Szauter P, Pardue M, Szostak J . Transfer of yeast telomeres to linear plasmids by recombination. Cell. 1984; 39(1):191-201. DOI: 10.1016/0092-8674(84)90205-8. View

17.

Hicks J, Hinnen A, Fink G . Properties of yeast transformation. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 2:1305-13. DOI: 10.1101/sqb.1979.043.01.149. View

18.

Boeke J, Lacroute F, Fink G . A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet. 1984; 197(2):345-6. DOI: 10.1007/BF00330984. View

19.

Chan C, Tye B . Autonomously replicating sequences in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1980; 77(11):6329-33. PMC: 350277. DOI: 10.1073/pnas.77.11.6329. View

20.

Dani G, Zakian V . Mitotic and meiotic stability of linear plasmids in yeast. Proc Natl Acad Sci U S A. 1983; 80(11):3406-10. PMC: 394052. DOI: 10.1073/pnas.80.11.3406. View