Current Strategies Using 3D Organoids to Establish Maternal-embryonic Interaction

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2024 Jun 4

PMID 38834510

Authors

Islam Mohamed Saadeldin

Seif Ehab

Ahmed Elsayed Noreldin

Ayman Abdel-Aziz Swelum

Seonggyu Bang

Hyejin Kim

Ki Young Yoon

Sanghoon Lee

Jongki Cho

Affiliations

Soon will be listed here.

Abstract

Importance: The creation of robust maternal-embryonic interactions and implantation models is important for comprehending the early stages of embryonic development and reproductive disorders. Traditional two-dimensional (2D) cell culture systems often fail to accurately mimic the highly complex conditions. The employment of three-dimensional (3D) organoids has emerged as a promising strategy to overcome these limitations in recent years. The advancements in the field of organoid technology have opened new avenues for studying the physiology and diseases affecting female reproductive tract.

Observations: This review summarizes the current strategies and advancements in the field of 3D organoids to establish maternal-embryonic interaction and implantation models for use in research and personalized medicine in assisted reproductive technology. The concepts of endometrial organoids, menstrual blood flow organoids, placental trophoblast organoids, stem cell-derived blastoids, and in vitro-generated embryo models are discussed in detail. We show the incorportaion of organoid systems and microfluidic technology to enhance tissue performance and precise management of the cellular surroundings.

Conclusions And Relevance: This review provides insights into the future direction of modeling maternal-embryonic interaction research and its combination with other powerful technologies to interfere with this dialogue either by promoting or hindering it for improving fertility or methods for contraception, respectively. The merging of organoid systems with microfluidics facilitates the creation of sophisticated and functional organoid models, enhancing insights into organ development, disease mechanisms, and personalized medical investigations.

Citing Articles

Establishment of an In Vitro Embryo-Endometrium Model Using Alginate-Embedded Mouse Embryos and Human Embryoid Body.

Kim Y, Kim Y, Kim J, Kim J, Kim S, Ku S Tissue Eng Regen Med. 2024; 22(1):77-89.

PMID: 39612135 PMC: 11711978. DOI: 10.1007/s13770-024-00682-w.

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