Single-strand Breakage of DNA in UV-irradiated UvrA, UvrB, and UvrC Mutants of Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Mar 1

PMID 3882671

Citations 2

Authors

M S Tang

L Ross

Affiliations

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Abstract

We transduced the uvrA6, uvrB5, uvrC34, and uvrC56 markers from the original mutagenized strains into an HF4714 background. Although in the original mutagenized strains uvrA6 cells are more UV sensitive than uvrB5 and uvrC34 cells, in the new background no significant difference in UV sensitivity is observed among uvrA6, uvrB5, and uvrC34 cells. No DNA single-strand breaks are detected in UV-irradiated uvrA6 or uvrB5 cells, whereas in contrast a significant number of single-strand breaks are detected in both UV-irradiated uvrC34 and uvrC56 cells. The number of single-strand breaks in these cells reaches a plateau at 20-J/m2 irradiation. Since these single-strand breaks can be detected by both alkaline sucrose and neutral formamide-sucrose gradient sedimentation, we concluded that the single-strand breaks observed in UV-irradiated uvrC cells are due to phosphodiester bond interruptions in DNA and are not due to apurinic/apyrimidinic sites.

Citing Articles

DNA-damaging activity of patulin in Escherichia coli.

Lee K, Roschenthaler R Appl Environ Microbiol. 1986; 52(5):1046-54.

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Nucleotide excision repair in Escherichia coli.

Van Houten B Microbiol Rev. 1990; 54(1):18-51.

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