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Genome-wide Identification Analysis in Wild-type Reveals Some Genes Defending Against

Overview
Journal Front Genet
Date 2024 May 30
PMID 38812971
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Abstract

exhibits strong resistance to late blight caused by but only an incomplete genome assembly based on short Illumina reads has been published. In this study, we generated the first chromosome-level draft genome for the wild-type potato species in China using Oxford Nanopore technology sequencing and Hi-C technology. The high-quality assembled genome size is 664 Mb with a scaffold N50 value of 49.17 Mb, of which 65.87% was occupied by repetitive sequences, and predominant long terminal repeats (42.51% of the entire genome). The genome of was predicted to contain 34,245 genes, of which 99.34% were functionally annotated. Moreover, 303 NBS-coding disease resistance (R) genes were predicted in the genome to investigate the potential mechanisms of resistance to late blight disease. The high-quality chromosome-level reference genome of is expected to provide potential valuable resources for intensively and effectively investigating molecular breeding and genetic research in the future.

References
1.
Letunic I, Bork P . Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res. 2016; 44(W1):W242-5. PMC: 4987883. DOI: 10.1093/nar/gkw290. View

2.
Rao S, Huntley M, Durand N, Stamenova E, Bochkov I, Robinson J . A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping. Cell. 2014; 159(7):1665-80. PMC: 5635824. DOI: 10.1016/j.cell.2014.11.021. View

3.
Karki H, Jansky S, Halterman D . Screening of Wild Potatoes Identifies New Sources of Late Blight Resistance. Plant Dis. 2020; 105(2):368-376. DOI: 10.1094/PDIS-06-20-1367-RE. View

4.
Nowicki M, Foolad M, Nowakowska M, Kozik E . Potato and Tomato Late Blight Caused by Phytophthora infestans: An Overview of Pathology and Resistance Breeding. Plant Dis. 2019; 96(1):4-17. DOI: 10.1094/PDIS-05-11-0458. View

5.
Langmead B, Salzberg S . Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012; 9(4):357-9. PMC: 3322381. DOI: 10.1038/nmeth.1923. View