Effect of Nonsense-mediated MRNA Decay Factor SMG9 Deficiency on Premature Aging in Zebrafish

Overview

Journal Commun Biol

Specialty Biology

Date 2024 May 28

PMID 38806677

Authors

Shaohong Lai

Hiroshi Shiraishi

Wulan Apridita Sebastian

Nobuyuki Shimizu

Ryohei Umeda

Mayo Ikeuchi

Kyoko Kiyota

Takashi Takeno

Shuya Miyazaki

Shinji Yano

Tatsuo Shimada

Akihiko Yoshimura

Reiko Hanada

Toshikatsu Hanada

Affiliations

Soon will be listed here.

Abstract

SMG9 is an essential component of the nonsense-mediated mRNA decay (NMD) machinery, a quality control mechanism that selectively degrades aberrant transcripts. Mutations in SMG9 are associated with heart and brain malformation syndrome (HBMS). However, the molecular mechanism underlying HBMS remains unclear. We generated smg9 mutant zebrafish (smg9) that have a lifespan of approximately 6 months or longer, allowing for analysis of the in vivo function of Smg9 in adults in more detail. smg9 zebrafish display congenital brain abnormalities and reduced cardiac contraction. Additionally, smg9 zebrafish exhibit a premature aging phenotype. Analysis of NMD target mRNAs shows a trend toward increased mRNA levels in smg9 zebrafish. Spermidine oxidase (Smox) is increased in smg9 zebrafish, resulting in the accumulation of byproducts, reactive oxygen species, and acrolein. The accumulation of smox mRNA due to NMD dysregulation caused by Smg9 deficiency leads to increased oxidative stress, resulting in premature aging.

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