MiR-329b-5p Affects Sheep Intestinal Epithelial Cells Against F17 Infection

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2024 May 24

PMID 38787178

Authors

Yeling Xu

Weihao Chen

Huiguo Yang

Zhenghai Song

Yeqing Wang

Rui Su

Joram M Mwacharo

Xiaoyang Lv

Wei Sun

Affiliations

Soon will be listed here.

Abstract

Diarrhea is the most common issue in sheep farms, typically due to pathogenic () infections, such as F17. microRNA, a primary type of non-coding RNA, has been shown to be involved in diarrhea caused by pathogenic . To elucidate the profound mechanisms of miRNA in F17 infections, methods such as F17 adhesion assay, colony counting assay, relative quantification of bacterial fimbriae gene expression, indirect immune fluorescence (IF), Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), Western blotting (WB), and scratch assay were conducted to investigate the effect of miR-329b-5p overexpression/knock-down on F17 susceptibility of sheep intestinal epithelial cells (IECs). The findings indicated that miR-329b-5p enhances the F17 resistance of sheep IECs to F17 by promoting adhesion between F17 and IEC, as well as IEC proliferation and migration. In summary, miR-329b-5p plays a crucial role in the defense of sheep IECs against F17 infection, providing valuable insights into its mechanism of action.

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