Comparative Assessment of CacyBP/SIP, β-catenin and Cannabinoid Receptors in the Adrenals of Hypertensive Rats

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2024 May 23

PMID 38780511

Authors

Magdalena Smereczanska

Natalia Domian

Alicja Lewandowska

Irena Kasacka

Affiliations

Soon will be listed here.

Abstract

Taking into account homeostatic disorders resulting from arterial hypertension and the key importance of CacyBP/SIP, β-catenin and endocannabinoids in the functioning of many organs, it was decided to assess the presence and distribution of CacyBP/SIP, β-catenin, CB1 and CB2 in the adrenal glands of hypertensive rats of various aetiology. The study was conducted on the adrenal glands of rats with spontaneous and renovascular hypertension. The expression of CacyBP/SIP, β-catenin, CB1 and CB2 was detected by immunohistochemistry and real-time PCR method. The results of the present study revealed both lower gene expression and immunoreactivity of CacyBP/SIP in the adrenal glands of all hypertensive groups compared to the normotensive rats. This study demonstrated a reduction in the immunoreactivity and expression of the β-catenin, CB1 and CB2 genes in the adrenals of 2K1C rats. While in SHR, the reaction showing β-catenin and CB1 was very weak or negative, and the expression of CB2 in the adrenal glands of these rats increased. The results of this study show, for the first time, marked differences in the expression of CacyBP/SIP, β-catenin and CB1 and CB2 cannabinoid receptors in the adrenal glands of rats with primary (SHR) and secondary hypertension (2K1C).

Citing Articles

Comparative assessment of CacyBP/SIP, β-catenin and cannabinoid receptors in the adrenals of hypertensive rats.

Smereczanska M, Domian N, Lewandowska A, Kasacka I J Cell Mol Med. 2024; 28(10):e18376.

PMID: 38780511 PMC: 11114211. DOI: 10.1111/jcmm.18376.

References

Toczek M, Schlicker E, Grzeda E, Malinowska B . Enhanced function of inhibitory presynaptic cannabinoid CB1 receptors on sympathetic nerves of DOCA-salt hypertensive rats. Life Sci. 2015; 138:78-85. DOI: 10.1016/j.lfs.2015.03.022. View

Micale V, Drago F . Endocannabinoid system, stress and HPA axis. Eur J Pharmacol. 2018; 834:230-239. DOI: 10.1016/j.ejphar.2018.07.039. View

Montecucco F, Lenglet S, Braunersreuther V, Burger F, Pelli G, Bertolotto M . CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion. J Mol Cell Cardiol. 2009; 46(5):612-20. DOI: 10.1016/j.yjmcc.2008.12.014. View

Batkai S, Pacher P, Osei-Hyiaman D, Radaeva S, Liu J, Harvey-White J . Endocannabinoids acting at cannabinoid-1 receptors regulate cardiovascular function in hypertension. Circulation. 2004; 110(14):1996-2002. PMC: 2756479. DOI: 10.1161/01.CIR.0000143230.23252.D2. View

Duan K, Mete O . Clinicopathologic Correlates of Primary Aldosteronism. Arch Pathol Lab Med. 2015; 139(7):948-54. DOI: 10.5858/arpa.2014-0156-RS. View

Funder J, Carey R, Mantero F, Murad M, Reincke M, Shibata H . The Management of Primary Aldosteronism: Case Detection, Diagnosis, and Treatment: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2016; 101(5):1889-916. DOI: 10.1210/jc.2015-4061. View

Pagotto U, Marsicano G, Cota D, Lutz B, Pasquali R . The emerging role of the endocannabinoid system in endocrine regulation and energy balance. Endocr Rev. 2005; 27(1):73-100. DOI: 10.1210/er.2005-0009. View

Xiao L, Xu B, Zhou L, Tan R, Zhou D, Fu H . Wnt/β-catenin regulates blood pressure and kidney injury in rats. Biochim Biophys Acta Mol Basis Dis. 2019; 1865(6):1313-1322. PMC: 6502648. DOI: 10.1016/j.bbadis.2019.01.027. View

Macheda M, Stacker S . Importance of Wnt signaling in the tumor stroma microenvironment. Curr Cancer Drug Targets. 2008; 8(6):454-65. DOI: 10.2174/156800908785699324. View

10.

Kusama K, Katayama Y, Oba K, Ishige T, Kebusa Y, Okazawa J . Expression of hard alpha-keratins in pilomatrixoma, craniopharyngioma, and calcifying odontogenic cyst. Am J Clin Pathol. 2005; 123(3):376-81. DOI: 10.1309/WVTR-R1DX-YMC8-PBMK. View

11.

Topolska-Wos A, Chazin W, Filipek A . CacyBP/SIP--Structure and variety of functions. Biochim Biophys Acta. 2015; 1860(1 Pt A):79-85. DOI: 10.1016/j.bbagen.2015.10.012. View

12.

Goral A, Bartkowska K, Djavadian R, Filipek A . CacyBP/SIP, a Hsp90 binding chaperone, in cellular stress response. Int J Biochem Cell Biol. 2018; 99:178-185. DOI: 10.1016/j.biocel.2018.04.012. View

13.

Lepicier P, Bouchard J, Lagneux C, Lamontagne D . Endocannabinoids protect the rat isolated heart against ischaemia. Br J Pharmacol. 2003; 139(4):805-15. PMC: 1573907. DOI: 10.1038/sj.bjp.0705313. View

14.

Kasacka I, Piotrowska Z, Domian N, Acewicz M, Lewandowska A . Canonical Wnt signaling in the kidney in different hypertension models. Hypertens Res. 2021; 44(9):1054-1066. DOI: 10.1038/s41440-021-00689-z. View

15.

Rimoldi S, Scherrer U, Messerli F . Secondary arterial hypertension: when, who, and how to screen?. Eur Heart J. 2013; 35(19):1245-54. DOI: 10.1093/eurheartj/eht534. View

16.

Smereczanska M, Domian N, Lewandowska A, Kasacka I . Comparative assessment of CacyBP/SIP, β-catenin and cannabinoid receptors in the adrenals of hypertensive rats. J Cell Mol Med. 2024; 28(10):e18376. PMC: 11114211. DOI: 10.1111/jcmm.18376. View

17.

Kasacka I, Piotrowska Z, Domian N, Lewandowska A . Wnt/β-catenin signaling in the adrenal glands of rats in various types of experimental hypertension. Endocr Connect. 2022; 11(8). PMC: 9346341. DOI: 10.1530/EC-22-0121. View

18.

Kearney P, Whelton M, Reynolds K, Muntner P, Whelton P, He J . Global burden of hypertension: analysis of worldwide data. Lancet. 2005; 365(9455):217-23. DOI: 10.1016/S0140-6736(05)17741-1. View

19.

Pacher P, Batkai S, Kunos G . Blood pressure regulation by endocannabinoids and their receptors. Neuropharmacology. 2005; 48(8):1130-8. PMC: 2225528. DOI: 10.1016/j.neuropharm.2004.12.005. View

20.

Matsuzawa S, Reed J . Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses. Mol Cell. 2001; 7(5):915-26. DOI: 10.1016/s1097-2765(01)00242-8. View