Polyphasic Approach and Potential Cyanotoxin Production by from the Río Grande De Comitán and Montebello Lakes National Park, Southern Mexico

Overview

Journal Int J Microbiol

Publisher Wiley

Specialty Microbiology

Date 2024 May 20

PMID 38764712

Authors

Javier Carmona Jimenez

Angela Caro Borrero

Aida Isabel Sanchez-Salas

Itzel Becerra-Absalon

Samuel Cires Gomez

Antonio Quesada Del Corral

Elvira Perona Urizar

David Ortiz Suarez

Marisa Mazari-Hiriart

Affiliations

Soon will be listed here.

Abstract

The development of anthropic activities during recent years has led to an increase in nutrient fluxes in the Río Grande de Comitán and Montebello Lakes National Park, Mexico. In turn, this has modified the dynamics of the biotic community, specifically favoring the presence of cyanobacteria tolerant to contamination. The continual and massive presence of species (spp.) in the system suggests a potential detrimental impact for economic issues and human health. In this study, we identify the morphological and molecular characteristics of populations from seven tropical (1,380-1,740 masl, 23.0-25.5°C) and calcareous lakes and two ponds from a water treatment plant. We also assess the ecological drivers that could be related to the presence of cyanotoxins in the system. The ecological preferences, morphology, 16S rRNA structure, and 16S-23S rRNA internal transcribed spacer found evidence for three species: distributed in neutral to slightly basic water (pH = 7.7-8.7), and and sp. in alkaline waters (pH = 9.1). The presence of the gene and its validation by liquid chromatography confirmed the presence of two microcystin variants (MC-RR and MC-LR) in at least three populations of . These microcystins put the health of the ecosystem and its inhabitants at risk, a condition that should be addressed and resolved with a water management and detoxification strategy in the basin.

References

Ranjbar M, Hamilton D, Etemad-Shahidi A, Helfer F . Individual-based modelling of cyanobacteria blooms: Physical and physiological processes. Sci Total Environ. 2021; 792:148418. DOI: 10.1016/j.scitotenv.2021.148418. View

Bertani G . Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol. 1951; 62(3):293-300. PMC: 386127. DOI: 10.1128/jb.62.3.293-300.1951. View

Salvador D, Churro C, Valerio E . Evaluating the influence of light intensity in mcyA gene expression and microcystin production in toxic strains of Planktothrix agardhii and Microcystis aeruginosa. J Microbiol Methods. 2016; 123:4-12. DOI: 10.1016/j.mimet.2016.02.002. View

Gao K, Li P, Watanabe T, Helbling E . COMBINED EFFECTS OF ULTRAVIOLET RADIATION AND TEMPERATURE ON MORPHOLOGY, PHOTOSYNTHESIS, AND DNA OF ARTHROSPIRA (SPIRULINA) PLATENSIS (CYANOPHYTA)(1). J Phycol. 2016; 44(3):777-86. DOI: 10.1111/j.1529-8817.2008.00512.x. View

Catherine A, Quiblier C, Yepremian C, Got P, Groleau A, Vincon-Leite B . Collapse of a Planktothrix agardhii perennial bloom and microcystin dynamics in response to reduced phosphate concentrations in a temperate lake. FEMS Microbiol Ecol. 2008; 65(1):61-73. DOI: 10.1111/j.1574-6941.2008.00494.x. View

Gaytan-Herrera M, Martinez-Almeida V, Oliva-Martinez M, Duran-Diaz A, Ramirez-Garcia P . Temporal variation of phytoplankton from the tropical reservoir Valle de Bravo, Mexico. J Environ Biol. 2011; 32(1):117-26. View

Gaget V, Welker M, Rippka R, de Marsac N . A polyphasic approach leading to the revision of the genus Planktothrix (Cyanobacteria) and its type species, P. agardhii, and proposal for integrating the emended valid botanical taxa, as well as three new species, Planktothrix paucivesiculata sp..... Syst Appl Microbiol. 2015; 38(3):141-58. DOI: 10.1016/j.syapm.2015.02.004. View

Wood S, Kelly L, Bouma-Gregson K, Humbert J, Laughinghouse 4th H, Lazorchak J . Toxic benthic freshwater cyanobacterial proliferations: Challenges and solutions for enhancing knowledge and improving monitoring and mitigation. Freshw Biol. 2021; 65(10):1824-1842. PMC: 8715960. DOI: 10.1111/fwb.13532. View

Barrios C, Nandini S, Sarma S . Effect of crude extracts from cyanobacterial blooms in Lake Texcoco (Mexico) on the population growth of Brachionus calyciflorus (Rotifera). Toxicon. 2017; 139:45-53. DOI: 10.1016/j.toxicon.2017.09.013. View

10.

Kumar S, Stecher G, Tamura K . MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016; 33(7):1870-4. PMC: 8210823. DOI: 10.1093/molbev/msw054. View

11.

Mateo P, Perona E, Berrendero E, Leganes F, Martin M, Golubic S . Life cycle as a stable trait in the evaluation of diversity of Nostoc from biofilms in rivers. FEMS Microbiol Ecol. 2011; 76(2):185-98. DOI: 10.1111/j.1574-6941.2010.01040.x. View

12.

Zamora-Barrios C, Nandini S, Sarma S . Bioaccumulation of microcystins in seston, zooplankton and fish: A case study in Lake Zumpango, Mexico. Environ Pollut. 2019; 249:267-276. DOI: 10.1016/j.envpol.2019.03.029. View

13.

Pineda-Mendoza R, Briones-Roblero C, Gonzalez-Escobedo R, Rivera-Orduna F, Martinez-Jeronimo F, Zuniga G . Seasonal changes in the bacterial community structure of three eutrophicated urban lakes in Mexico city, with emphasis on Microcystis spp. Toxicon. 2020; 179:8-20. DOI: 10.1016/j.toxicon.2020.02.019. View

14.

Yema L, Litchman E, de Tezanos Pinto P . The role of heterocytes in the physiology and ecology of bloom-forming harmful cyanobacteria. Harmful Algae. 2017; 60:131-138. DOI: 10.1016/j.hal.2016.11.007. View

15.

Briand E, Yepremian C, Humbert J, Quiblier C . Competition between microcystin- and non-microcystin-producing Planktothrix agardhii (cyanobacteria) strains under different environmental conditions. Environ Microbiol. 2008; 10(12):3337-48. DOI: 10.1111/j.1462-2920.2008.01730.x. View

16.

Favari L, Lopez E, Martinez-Tabche L, Diaz-Pardo E . Effect of insecticides on plankton and fish of Ignacio Ramirez reservoir (Mexico): a biochemical and biomagnification study. Ecotoxicol Environ Saf. 2002; 51(3):177-86. DOI: 10.1006/eesa.2002.2142. View

17.

Yanez-Montalvo A, Aguila B, Gomez-Acata E, Guerrero-Jacinto M, Oseguera L, Falcon L . Shifts in water column microbial composition associated to lakes with different trophic conditions: "Lagunas de Montebello" National Park, Chiapas, México. PeerJ. 2022; 10:e13999. PMC: 9484458. DOI: 10.7717/peerj.13999. View

18.

Pancrace C, Barny M, Ueoka R, Calteau A, Scalvenzi T, Pedron J . Insights into the Planktothrix genus: Genomic and metabolic comparison of benthic and planktic strains. Sci Rep. 2017; 7:41181. PMC: 5259702. DOI: 10.1038/srep41181. View

19.

Vasconcelos V, Martins A, Vale M, Antunes A, Azevedo J, Welker M . First report on the occurrence of microcystins in planktonic cyanobacteria from Central Mexico. Toxicon. 2010; 56(3):425-31. DOI: 10.1016/j.toxicon.2010.04.011. View

20.

Shimura Y, Fujisawa T, Hirose Y, Misawa N, Kanesaki Y, Nakamura Y . Complete sequence and structure of the genome of the harmful algal bloom-forming cyanobacterium Planktothrix agardhii NIES-204 and detailed analysis of secondary metabolite gene clusters. Harmful Algae. 2021; 101:101942. DOI: 10.1016/j.hal.2020.101942. View