Review of Fish Protein Hydrolysates: Production Methods, Antioxidant and Antimicrobial Activity and Nanoencapsulation

Overview

Journal Food Sci Biotechnol

Specialty Biotechnology

Date 2024 May 16

PMID 38752116

Authors

Mahrokh Nemati

Seyed Rasoul Shahosseini

Peiman Ariaii

Affiliations

Soon will be listed here.

Abstract

Marine products have gained popularity due to their valuable components, especially protein, despite generating significant waste. Protein hydrolysates are widely recognized as the most effective method for transforming these low-value raw materials into high-value products. Fish protein hydrolysate (FPH), sourced from various aquatic wastes such as bones, scales, skin, and others, is rich in protein for value-added products. However, the hydrophobic peptides have limitations like an unpleasant taste and high solubility. Microencapsulation techniques provide a scientific approach to address these limitations and safeguard bioactive peptides. This review examines current research on FPH production methods and their antioxidant and antibacterial activities. Enzymatic hydrolysis using commercial enzymes is identified as the optimal method, and the antioxidant and antibacterial properties of FPH are substantiated. Microencapsulation using nanoliposomes effectively extends the inhibitory activity and enhances antioxidant and antibacterial capacities. Nevertheless, more research is needed to mitigate the bitter taste associated with FPH and enhance sensory attributes.

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References

Ramezanzade L, Hosseini S, Nikkhah M . Biopolymer-coated nanoliposomes as carriers of rainbow trout skin-derived antioxidant peptides. Food Chem. 2017; 234:220-229. DOI: 10.1016/j.foodchem.2017.04.177. View

Krobthong S, Yingchutrakul Y, Visessanguan W, Mahatnirunkul T, Samutrtai P, Chaichana C . Study of the Lipolysis Effect of Nanoliposome-Encapsulated Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach. Foods. 2021; 10(9). PMC: 8468392. DOI: 10.3390/foods10092157. View

Liu B, Li N, Chen F, Zhang J, Sun X, Xu L . Review on the release mechanism and debittering technology of bitter peptides from protein hydrolysates. Compr Rev Food Sci Food Saf. 2022; 21(6):5153-5170. DOI: 10.1111/1541-4337.13050. View

Kristinsson H, Rasco B . Fish protein hydrolysates: production, biochemical, and functional properties. Crit Rev Food Sci Nutr. 2000; 40(1):43-81. DOI: 10.1080/10408690091189266. View

Jamshidi A, Antequera T, Solomando J, Perez-Palacios T . Microencapsulation of oil and protein hydrolysate from fish within a high-pressure homogenized double emulsion. J Food Sci Technol. 2020; 57(1):60-69. PMC: 6952508. DOI: 10.1007/s13197-019-04029-5. View

Ovissipour M, Rasco B, Shiroodi S, Modanlow M, Gholami S, Nemati M . Antioxidant activity of protein hydrolysates from whole anchovy sprat (Clupeonella engrauliformis) prepared using endogenous enzymes and commercial proteases. J Sci Food Agric. 2012; 93(7):1718-26. DOI: 10.1002/jsfa.5957. View

Vazquez J, Blanco M, Massa A, Amado I, Perez-Martin R . Production of Fish Protein Hydrolysates from Scyliorhinus canicula Discards with Antihypertensive and Antioxidant Activities by Enzymatic Hydrolysis and Mathematical Optimization Using Response Surface Methodology. Mar Drugs. 2017; 15(10). PMC: 5666414. DOI: 10.3390/md15100306. View

Cheung R, Ng T, Wong J . Marine Peptides: Bioactivities and Applications. Mar Drugs. 2015; 13(7):4006-43. PMC: 4515606. DOI: 10.3390/md13074006. View

Tejpal C, Vijayagopal P, Elavarasan K, Linga Prabu D, Lekshmi R, Asha K . Antioxidant, functional properties and amino acid composition of pepsin-derived protein hydrolysates from whole tilapia waste as influenced by pre-processing ice storage. J Food Sci Technol. 2017; 54(13):4257-4267. PMC: 5686006. DOI: 10.1007/s13197-017-2897-9. View

10.

Ennaas N, Hammami R, Beaulieu L, Fliss I . Purification and characterization of four antibacterial peptides from protamex hydrolysate of Atlantic mackerel (Scomber scombrus) by-products. Biochem Biophys Res Commun. 2015; 462(3):195-200. DOI: 10.1016/j.bbrc.2015.04.091. View

11.

Hosseini S, Ramezanzade L, Nikkhah M . Nano-liposomal entrapment of bioactive peptidic fraction from fish gelatin hydrolysate. Int J Biol Macromol. 2017; 105(Pt 2):1455-1463. DOI: 10.1016/j.ijbiomac.2017.05.141. View

12.

Jiang H, Tong T, Sun J, Xu Y, Zhao Z, Liao D . Purification and characterization of antioxidative peptides from round scad (Decapterus maruadsi) muscle protein hydrolysate. Food Chem. 2014; 154:158-63. DOI: 10.1016/j.foodchem.2013.12.074. View

13.

Bi J, Tian C, Jiang J, Zhang G, Hao H, Hou H . Antibacterial Activity and Potential Application in Food Packaging of Peptides Derived from Turbot Viscera Hydrolysate. J Agric Food Chem. 2020; 68(37):9968-9977. DOI: 10.1021/acs.jafc.0c03146. View

14.

McClements D, Ozturk B . Utilization of Nanotechnology to Improve the Handling, Storage and Biocompatibility of Bioactive Lipids in Food Applications. Foods. 2021; 10(2). PMC: 7915003. DOI: 10.3390/foods10020365. View

15.

Blanco M, Vazquez J, Perez-Martin R, Sotelo C . Hydrolysates of Fish Skin Collagen: An Opportunity for Valorizing Fish Industry Byproducts. Mar Drugs. 2017; 15(5). PMC: 5450537. DOI: 10.3390/md15050131. View

16.

Wald M, Schwarz K, Rehbein H, Bussmann B, Beermann C . Detection of antibacterial activity of an enzymatic hydrolysate generated by processing rainbow trout by-products with trout pepsin. Food Chem. 2016; 205:221-8. DOI: 10.1016/j.foodchem.2016.03.002. View

17.

Burey P, Bhandari B, Howes T, Gidley M . Hydrocolloid gel particles: formation, characterization, and application. Crit Rev Food Sci Nutr. 2008; 48(5):361-77. DOI: 10.1080/10408390701347801. View

18.

Pavlovic N, Mijalkovic J, dordevic V, Pecarski D, Bugarski B, Knezevic-Jugovic Z . Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and digestion. Food Chem X. 2022; 15:100370. PMC: 9240801. DOI: 10.1016/j.fochx.2022.100370. View

19.

Otchere E, McKay B, English M, Aryee A . Current trends in nano-delivery systems for functional foods: a systematic review. PeerJ. 2023; 11:e14980. PMC: 10026715. DOI: 10.7717/peerj.14980. View

20.

Pezeshk S, Ojagh S, Rezaei M, Shabanpour B . Fractionation of Protein Hydrolysates of Fish Waste Using Membrane Ultrafiltration: Investigation of Antibacterial and Antioxidant Activities. Probiotics Antimicrob Proteins. 2018; 11(3):1015-1022. DOI: 10.1007/s12602-018-9483-y. View