Detection of C Polysaccharide in Streptococcus Pneumoniae in the Sputa of Pneumonia Patients by an Enzyme-linked Immunosorbent Assay

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1985 Jul 1

PMID 3874879

Citations 16

Authors

H Holmberg

T Holme

A Krook

T Olsson

L Sjoberg

A M Sjogren

Affiliations

Soon will be listed here.

Abstract

The pneumococcal C polysaccharide (PnC) is species specific and believed to be a cell wall component of all pneumococcal types. A sandwich enzyme-linked immunosorbent assay (ELISA) for detection of PnC in sputa has been developed by using a monoclonal antiphosphorylcholine antibody and a polyclonal rabbit anti-PnC antiserum in the test system. A 1-year study of adult hospitalized patients with community-acquired pneumonia was performed. A total of 147 patients with clinical and radiological evidence for pneumonia were accepted for the study. Of these, 105 patients provided a sputum sample upon admission to the ward. The sputa were cultured semiquantitatively as well as tested for the presence of antigen. Of the sputum samples from patients with Streptococcus pneumoniae, 27 of 33 (accounting for a sensitivity of 82%) were positive in the ELISA test. Of the sputum samples from patients with pneumonia of some other known or suspected etiology, 32 of 34 (accounting for a specificity of 94%) were negative. In addition, 7 sputum samples from 31 patients with pneumonia of unknown etiology were positive. The ELISA test described here is in our opinion a sensitive and specific test for detecting PnC from S. pneumoniae in sputa from patients with untreated pneumonia.

Citing Articles

Highly penicillin-resistant multidrug-resistant pneumococcus-like strains colonizing children in Oeiras, Portugal: genomic characteristics and implications for surveillance.

Simoes A, Sa-Leao R, Eleveld M, Tavares D, Carrico J, Bootsma H J Clin Microbiol. 2009; 48(1):238-46.

PMID: 19906899 PMC: 2812262. DOI: 10.1128/JCM.01313-09.

Diagnosis of Streptococcus pneumoniae pneumonia by quantitative enzyme linked immunosorbent assay of C-polysaccharide antigen.

Gillespie S, Smith M, Dickens A, Raynes J, McAdam K J Clin Pathol. 1994; 47(8):749-51.

PMID: 7962631 PMC: 502151. DOI: 10.1136/jcp.47.8.749.

Determination of antibodies to pneumococcal C polysaccharide in patients with community-acquired pneumonia.

Holmberg H, Krook A, Sjogren A J Clin Microbiol. 1985; 22(5):808-14.

PMID: 4056007 PMC: 268532. DOI: 10.1128/jcm.22.5.808-814.1985.

Diagnosis of pneumococcal pneumonia by detection of antigen in saliva.

Krook A, Fredlund H, Holmberg H Eur J Clin Microbiol. 1986; 5(6):639-42.

PMID: 3803376 DOI: 10.1007/BF02013288.

Comparison of enzyme-linked immunosorbent assay with coagglutination and latex agglutination for rapid diagnosis of pneumococcal pneumonia by detecting antigen in sputa.

Holmberg H, Krook A Eur J Clin Microbiol. 1986; 5(3):282-6.

PMID: 3743553 DOI: 10.1007/BF02017782.

References

Leach R, Coonrod J . Detection of penumococcal antigens in the sputum in pneumococcal pneumonia. Am Rev Respir Dis. 1977; 116(5):847-51. DOI: 10.1164/arrd.1977.116.5.847. View

Spencer R, Savage M . Use of Counter and rocket immunoelectrophoresis in acute respiratory infections due to Streptococcus pneumoniae. J Clin Pathol. 1976; 29(3):187-90. PMC: 476019. DOI: 10.1136/jcp.29.3.187. View

Bartlett J, Finegold S . Bacteriology of expectorated sputum with quantitative culture and wash technique compared to transtracheal aspirates. Am Rev Respir Dis. 1978; 117(6):1019-27. DOI: 10.1164/arrd.1978.117.6.1019. View

Trollfors B, Berntsson E, Elgefors B, Kaijser B . Counterimmunoelectrophoresis of sputum and blood for the diagnosis of chest infections caused by pneumococci or Haemophilus influenzae. Scand J Infect Dis. 1979; 11(1):31-4. DOI: 10.3109/inf.1979.11.issue-1.04. View

Poxton I, Tarelli E, BADDILEY J . The structure of C-polysaccharide from the walls of Streptococcus pneumoniae. Biochem J. 1978; 175(3):1033-42. PMC: 1186166. DOI: 10.1042/bj1751033. View

Nowinski R, Lostrom M, Tam M, Stone M, Burnette W . The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses. Virology. 1979; 93(1):111-26. DOI: 10.1016/0042-6822(79)90280-0. View

Edwards E, Coonrod J . Coagglutination and counterimmunoelectrophoresis for detection of pneumococcal antigens in the sputum of pneumonia patients. J Clin Microbiol. 1980; 11(5):488-91. PMC: 273438. DOI: 10.1128/jcm.11.5.488-491.1980. View

Jennings H, Lugowski C, Young N . Structure of the complex polysaccharide C-substance from Streptococcus pneumoniae type 1. Biochemistry. 1980; 19(20):4712-9. DOI: 10.1021/bi00561a026. View

Gearhart P, Johnson N, Douglas R, Hood L . IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts. Nature. 1981; 291(5810):29-34. DOI: 10.1038/291029a0. View

10.

CLAFLIN J, Hudak S, Maddalena A . Anti-phosphocholine hybridoma antibodies. I. Direct evidence for three distinct families of antibodies in the murine response. J Exp Med. 1981; 153(2):352-64. PMC: 2186073. DOI: 10.1084/jem.153.2.352. View

11.

Kalin M, Lindberg A, Olausson E . Diagnosis of pneumococcal pneumonia by coagglutination and counterimmunoelectrophoresis of sputum samples. Eur J Clin Microbiol. 1982; 1(2):91-6. DOI: 10.1007/BF02014198. View

12.

Kalin M . Bacteremic pneumococcal pneumonia: value of culture of nasopharyngeal specimens and examination of washed sputum specimens. Eur J Clin Microbiol. 1982; 1(6):394-6. DOI: 10.1007/BF02019941. View

13.

Szu S, Clarke S, Robbins J . Protection against pneumococcal infection in mice conferred by phosphocholine-binding antibodies: specificity of the phosphocholine binding and relation to several types. Infect Immun. 1983; 39(2):993-9. PMC: 348048. DOI: 10.1128/iai.39.2.993-999.1983. View

14.

Pedersen F, HENRICHSEN J, Sorensen U, Nielsen J . Anti-C-carbohydrate antibodies after pneumococcal vaccination. Acta Pathol Microbiol Immunol Scand C. 1982; 90(6):353-5. DOI: 10.1111/j.1699-0463.1982.tb01462.x. View

15.

Kalin M, Lindberg A, Tunevall G . Etiological diagnosis of bacterial pneumonia by gram stain and quantitative culture of expectorates. Leukocytes or alveolar macrophages as indicators of sample representativity. Scand J Infect Dis. 1983; 15(2):153-60. DOI: 10.3109/inf.1983.15.issue-2.05. View

16.

Kalin M, Lindberg A . Diagnosis of pneumococcal pneumonia: a comparison between microscopic examination of expectorate, antigen detection and cultural procedures. Scand J Infect Dis. 1983; 15(3):247-55. DOI: 10.3109/inf.1983.15.issue-3.04. View

17.

Sorensen U, Agger R, Bennedsen J, HENRICHSEN J . Phosphorylcholine determinants in six pneumococcal capsular polysaccharides detected by monoclonal antibody. Infect Immun. 1984; 43(3):876-8. PMC: 264264. DOI: 10.1128/iai.43.3.876-878.1984. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

19.

Austrian R, Gold J . PNEUMOCOCCAL BACTEREMIA WITH ESPECIAL REFERENCE TO BACTEREMIC PNEUMOCOCCAL PNEUMONIA. Ann Intern Med. 1964; 60:759-76. DOI: 10.7326/0003-4819-60-5-759. View

20.

Schiffman G, Bornstein D, Austrian R . Capsulation of pneumococcus with soluble cell wall-like polysaccharide. II. Nonidentity of cell wall and soluble cell wall-like polysaccharides derived from the same and from different pneumococcal strains. J Exp Med. 1971; 134(3 Pt 1):600-17. PMC: 2139069. DOI: 10.1084/jem.134.3.600. View