Relationships Among Hydrogen Peroxide Concentration, Catalase, Glucose Oxidase, and Antimicrobial Activities of Honeys

Overview

Journal Foods

Specialty Biotechnology

Date 2024 May 11

PMID 38731715

Authors

Sandra M Oses

Carlos Rodriguez

Olga Valencia

Miguel A Fernandez-Muino

M Teresa Sancho

Affiliations

Soon will be listed here.

Abstract

Honey is a natural sweetener made by bees that exhibits antimicrobial activity, mainly related to its HO content. The aim of this work was to research the HO concentration of 24 Spanish honeys from different botanical origins, studying their possible correlation with glucose oxidase (GOx), catalase (CAT), and anti- activities (minimal inhibition concentration (MIC), minimal bactericidal concentration (MBC), and percentage of inhibition at 5% (/) honey against ), as well as possible correlations among all the analyzed parameters. The results showed that the HO concentration did not depend on the botanical origin of the honeys. There were neither correlations between the HO concentration and the activities of GOx and CAT, nor between GOx and antimicrobial activity. However, CAT and antimicrobial activities were positively correlated. Therefore, CAT could be successfully used as a possible marker of the antimicrobial activity of honeys against . Furthermore, a linear regression model has been fitted to explain the antimicrobial activity from CAT and GOx activity and HO concentration. Although HO is one of the compounds involved in honey's antibacterial activity, this capacity also strongly depends on other honey components (such as low water activity, acidity, osmolarity, and phenolic compounds). The very high anti- activity exhibited by all samples could be interesting for commercial honey-based formulations also helping to promote local beekeeping.

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References

Dunford C, Cooper R, Molan P, White R . The use of honey in wound management. Nurs Stand. 2002; 15(11):63-8. DOI: 10.7748/ns2000.11.15.11.63.c2952. View

Lin T, Huang L, Cheng N, Wang Y, Ning Z, Huang S . The in vitro and in vivo antibacterial activities of uniflorous honey from a medicinal plant, Scrophularia ningpoensis Hemsl., and characterization of its chemical profile with UPLC-MS/MS. J Ethnopharmacol. 2022; 296:115499. DOI: 10.1016/j.jep.2022.115499. View

Nolan V, Harrison J, Cox J . Dissecting the Antimicrobial Composition of Honey. Antibiotics (Basel). 2019; 8(4). PMC: 6963415. DOI: 10.3390/antibiotics8040251. View

Bucekova M, Jardekova L, Juricova V, Bugarova V, Di Marco G, Gismondi A . Antibacterial Activity of Different Blossom Honeys: New Findings. Molecules. 2019; 24(8). PMC: 6514785. DOI: 10.3390/molecules24081573. View

Moussa A, Noureddine D, Mohamed H, Abdelmelek M, Saad A . Antibacterial activity of various honey types of Algeria against Staphylococcus aureus and Streptococcus pyogenes. Asian Pac J Trop Med. 2012; 5(10):773-6. DOI: 10.1016/S1995-7645(12)60141-2. View

Wang H, Li L, Lin X, Bai W, Xiao G, Liu G . Composition, functional properties and safety of honey: a review. J Sci Food Agric. 2023; 103(14):6767-6779. DOI: 10.1002/jsfa.12720. View

White Jr J, SUBERS M, SCHEPARTZ A . The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system. Biochim Biophys Acta. 1963; 73:57-70. DOI: 10.1016/0006-3002(63)90359-7. View

Huidobro J, Sanchez M, Muniategui S, Sancho M . Precise method for the measurement of catalase activity in honey. J AOAC Int. 2005; 88(3):800-4. View

Faundez X, Baez M, Martinez J, Zuniga-Lopez M, Espinoza J, Fuentes E . Evaluation of the generation of reactive oxygen species and antibacterial activity of honey as a function of its phenolic and mineral composition. Food Chem. 2023; 426:136561. DOI: 10.1016/j.foodchem.2023.136561. View

10.

Bucekova M, Godocikova J, Gueyte R, Chambrey C, Majtan J . Characterisation of physicochemical parameters and antibacterial properties of New Caledonian honeys. PLoS One. 2023; 18(10):e0293730. PMC: 10617706. DOI: 10.1371/journal.pone.0293730. View

11.

Majtan J . Honey: an immunomodulator in wound healing. Wound Repair Regen. 2014; 22(2):187-92. DOI: 10.1111/wrr.12117. View

12.

Brudzynski K . Effect of hydrogen peroxide on antibacterial activities of Canadian honeys. Can J Microbiol. 2007; 52(12):1228-37. DOI: 10.1139/w06-086. View

13.

Godocikova J, Bugarova V, Kast C, Majtan V, Majtan J . Antibacterial potential of Swiss honeys and characterisation of their bee-derived bioactive compounds. J Sci Food Agric. 2019; 100(1):335-342. DOI: 10.1002/jsfa.10043. View

14.

Subrahmanyam M . Topical application of honey for burn wound treatment - an overview. Ann Burns Fire Disasters. 2011; 20(3):137-9. PMC: 3188068. View

15.

Tsavea E, Vardaka F, Savvidaki E, Kellil A, Kanelis D, Bucekova M . Physicochemical Characterization and Biological Properties of Pine Honey Produced across Greece. Foods. 2022; 11(7). PMC: 8997407. DOI: 10.3390/foods11070943. View

16.

Bugarova V, Godocikova J, Bucekova M, Brodschneider R, Majtan J . Effects of the Carbohydrate Sources Nectar, Sucrose and Invert Sugar on Antibacterial Activity of Honey and Bee-Processed Syrups. Antibiotics (Basel). 2021; 10(8). PMC: 8388957. DOI: 10.3390/antibiotics10080985. View

17.

Alevia M, Rasines S, Cantero L, Sancho M, Fernandez-Muino M, Oses S . Chemical Extraction and Gastrointestinal Digestion of Honey: Influence on Its Antioxidant, Antimicrobial and Anti-Inflammatory Activities. Foods. 2021; 10(6). PMC: 8235604. DOI: 10.3390/foods10061412. View

18.

Bucekova M, Valachova I, Kohutova L, Prochazka E, Klaudiny J, Majtan J . Honeybee glucose oxidase--its expression in honeybee workers and comparative analyses of its content and H2O2-mediated antibacterial activity in natural honeys. Naturwissenschaften. 2014; 101(8):661-70. DOI: 10.1007/s00114-014-1205-z. View

19.

Almasaudi S . The antibacterial activities of honey. Saudi J Biol Sci. 2021; 28(4):2188-2196. PMC: 8071826. DOI: 10.1016/j.sjbs.2020.10.017. View

20.

Bucekova M, Buriova M, Pekarik L, Majtan V, Majtan J . Phytochemicals-mediated production of hydrogen peroxide is crucial for high antibacterial activity of honeydew honey. Sci Rep. 2018; 8(1):9061. PMC: 5998132. DOI: 10.1038/s41598-018-27449-3. View