XA21-mediated Resistance to Pv. is Dose Dependent

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2024 May 10

PMID 38726377

Authors

Nan Zhang

Xiaoou Dong

Rashmi Jain

Deling Ruan

Artur Teixeira de Araujo Junior

Yan Li

Anna Lipzen

Joel Martin

Kerrie Barry

Pamela C Ronald

Affiliations

Soon will be listed here.

Abstract

The rice receptor kinase XA21 confers broad-spectrum resistance to pv. (), the causal agent of rice bacterial blight disease. To investigate the relationship between the expression level of and resulting resistance, we generated independent transgenic rice lines accumulating the XA21 immune receptor fused with an HA epitope tag. Whole-genome sequence analysis identified the T-DNA insertion sites in sixteen independent T0 events. Through quantification of the HA-XA21 protein and assessment of the resistance to strain PXO99 in six independent transgenic lines, we observed that XA21-mediated resistance is dose dependent. In contrast, based on the four agronomic traits quantified in these experiments, yield is unlikely to be affected by the expression level of . These findings extend our knowledge of XA21-mediated defense and contribute to the growing number of well-defined genomic landing pads in the rice genome that can be targeted for gene insertion without compromising yield.

Citing Articles

The Role of Genetic Resistance in Rice Disease Management.

Danso Ofori A, Zheng T, Titriku J, Appiah C, Xiang X, Kandhro A Int J Mol Sci. 2025; 26(3).

PMID: 39940724 PMC: 11817016. DOI: 10.3390/ijms26030956.

References

Zhai W, Li X, Tian W, Zhou Y, Pan X, Cao S . Introduction of a rice blight resistance gene,Xa21, into five Chinese rice varieties through an Agrobacterium-mediated system. Sci China C Life Sci. 2008; 43(4):361-8. DOI: 10.1007/BF02879300. View

Altpeter F, Springer N, Bartley L, Blechl A, Brutnell T, Citovsky V . Advancing Crop Transformation in the Era of Genome Editing. Plant Cell. 2016; 28(7):1510-20. PMC: 4981132. DOI: 10.1105/tpc.16.00196. View

Peng Y, Bartley L, Chen X, Dardick C, Chern M, Ruan R . OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice. Mol Plant. 2009; 1(3):446-58. DOI: 10.1093/mp/ssn024. View

Zhou J, Zhang Y . Plant Immunity: Danger Perception and Signaling. Cell. 2020; 181(5):978-989. DOI: 10.1016/j.cell.2020.04.028. View

Boutrot F, Segonzac C, Chang K, Qiao H, Ecker J, Zipfel C . Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1. Proc Natl Acad Sci U S A. 2010; 107(32):14502-7. PMC: 2922558. DOI: 10.1073/pnas.1003347107. View

Dean C, Jones J, Favreau M, Dunsmuir P, Bedbrook J . Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants. Nucleic Acids Res. 1988; 16(19):9267-83. PMC: 338705. DOI: 10.1093/nar/16.19.9267. View

Huang Y, Minaker S, Roth C, Huang S, Hieter P, Lipka V . An E4 ligase facilitates polyubiquitination of plant immune receptor resistance proteins in Arabidopsis. Plant Cell. 2014; 26(1):485-96. PMC: 3963591. DOI: 10.1105/tpc.113.119057. View

Zhai W, Chen C, Zhu X, Chen X, Zhang D, Li X . Analysis of T-DNA- Xa21 loci and bacterial blight resistance effects of the transgene Xa21 in transgenic rice. Theor Appl Genet. 2004; 109(3):534-42. DOI: 10.1007/s00122-004-1670-4. View

Li Y, Dong O, Johnson K, Zhang Y . MOS1 epigenetically regulates the expression of plant Resistance gene SNC1. Plant Signal Behav. 2011; 6(3):434-6. PMC: 3142431. DOI: 10.4161/psb.6.3.14495. View

10.

Ercoli M, Luu D, Rim E, Shigenaga A, de Araujo Jr A, Chern M . Plant immunity: Rice XA21-mediated resistance to bacterial infection. Proc Natl Acad Sci U S A. 2022; 119(8). PMC: 8872720. DOI: 10.1073/pnas.2121568119. View

11.

Yang L, Ding J, Zhang C, Jia J, Weng H, Liu W . Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR. Plant Cell Rep. 2004; 23(10-11):759-63. DOI: 10.1007/s00299-004-0881-0. View

12.

Mumm R . A look at product development with genetically modified crops: examples from maize. J Agric Food Chem. 2013; 61(35):8254-9. DOI: 10.1021/jf400685y. View

13.

Matzke A, Matzke M . Position effects and epigenetic silencing of plant transgenes. Curr Opin Plant Biol. 1999; 1(2):142-8. DOI: 10.1016/s1369-5266(98)80016-2. View

14.

Dong O, Yu S, Jain R, Zhang N, Duong P, Butler C . Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9. Nat Commun. 2020; 11(1):1178. PMC: 7055238. DOI: 10.1038/s41467-020-14981-y. View

15.

Xu F, Cheng Y, Kapos P, Huang Y, Li X . P-loop-dependent NLR SNC1 can oligomerize and activate immunity in the nucleus. Mol Plant. 2014; 7(12):1801-4. DOI: 10.1093/mp/ssu097. View

16.

Huang S, Monaghan J, Zhong X, Lin L, Sun T, Dong O . HSP90s are required for NLR immune receptor accumulation in Arabidopsis. Plant J. 2014; 79(3):427-39. DOI: 10.1111/tpj.12573. View

17.

Bollinedi H, Krishnan S G, Prabhu K, Singh N, Mishra S, Khurana J . Molecular and Functional Characterization of GR2-R1 Event Based Backcross Derived Lines of Golden Rice in the Genetic Background of a Mega Rice Variety Swarna. PLoS One. 2017; 12(1):e0169600. PMC: 5221763. DOI: 10.1371/journal.pone.0169600. View

18.

Zhang Y, Goritschnig S, Dong X, Li X . A gain-of-function mutation in a plant disease resistance gene leads to constitutive activation of downstream signal transduction pathways in suppressor of npr1-1, constitutive 1. Plant Cell. 2003; 15(11):2636-46. PMC: 280567. DOI: 10.1105/tpc.015842. View

19.

Caddell D, Park C, Thomas N, Canlas P, Ronald P . Silencing of the Rice Gene LRR1 Compromises Rice Xa21 Transcript Accumulation and XA21-Mediated Immunity. Rice (N Y). 2017; 10(1):23. PMC: 5440417. DOI: 10.1186/s12284-017-0162-5. View

20.

Lu D, Lin W, Gao X, Wu S, Cheng C, Avila J . Direct ubiquitination of pattern recognition receptor FLS2 attenuates plant innate immunity. Science. 2011; 332(6036):1439-42. PMC: 3243913. DOI: 10.1126/science.1204903. View