Deciphering the TET3 Interactome in Primary Thymic Developing T cells

Overview

Journal iScience

Publisher Cell Press

Date 2024 May 7

PMID 38711449

Authors

Dimitris Theofilatos

Tricia Ho

Greg Waitt

Tarmo Aijo

Lucio M Schiapparelli

Erik J Soderblom

Ageliki Tsagaratou

Affiliations

Soon will be listed here.

Abstract

Ten-eleven translocation (TET) proteins are DNA dioxygenases that mediate active DNA demethylation. TET3 is the most highly expressed TET protein in thymic developing T cells. TET3, either independently or in cooperation with TET1 or TET2, has been implicated in T cell lineage specification by regulating DNA demethylation. However, TET-deficient mice exhibit complex phenotypes, suggesting that TET3 exerts multifaceted roles, potentially by interacting with other proteins. We performed liquid chromatography with tandem mass spectrometry in primary developing T cells to identify TET3 interacting partners in endogenous, conditions. We discover TET3 interacting partners. Our data establish that TET3 participates in a plethora of fundamental biological processes, such as transcriptional regulation, RNA polymerase elongation, splicing, DNA repair, and DNA replication. This resource brings in the spotlight emerging functions of TET3 and sets the stage for systematic studies to dissect the precise mechanistic contributions of TET3 in shaping T cell biology.

Citing Articles

Predicting differentially methylated cytosines in TET and DNMT3 knockout mutants via a large language model.

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Concomitant loss of TET2 and TET3 results in T cell expansion and genomic instability in mice.

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