Effects of Monovalent and Divalent Cations on the Rheology of Entangled DNA

Overview

Journal Soft Matter

Publisher Royal Society of Chemistry

Specialties Biochemistry
Chemistry

Date 2024 Apr 30

PMID 38686506

Authors

Jennifer Harnett

Simon Weir

Davide Michieletto

Affiliations

Soon will be listed here.

Abstract

In this paper we investigate the effects of varying cation valency and concentration on the rheology of entangled DNA solutions. We show that monovalent cations moderately increase the viscoelasticty of the solutions mainly by stabilising linear concatenation of DNA "monomers" hybridisation of their sticky ends. On the contrary, divalent cations have a far more complex and dramatic effect on the rheology of the solution and we observe evidence of inter-molecular DNA-DNA bridging by Mg. We argue that these results may be interesting in the context of dense solutions of single and double stranded DNA, or in biotechnology applications such as DNA origami and DNA hydrogels.

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