Stauntonia Chinensis Injection Relieves Neuropathic Pain by Increasing the Expression of PSD-95 and Reducing the Proliferation of Phagocytic Microglia

Overview

Journal Ibrain

Specialty Neurology

Date 2024 Apr 29

PMID 38682013

Authors

Wenwen Deng

Helin Zou

Li Qian

Senio Campos de Souza

Qian Chen

Song Cao

Affiliations

Soon will be listed here.

Abstract

Neuroinflammation induced by engulfment of synapses by phagocytic microglia plays a crucial role in neuropathic pain. Stauntonia chinensis is extracted from DC, which has been used as a traditional Chinese medicine to control trigeminal neuralgia or sciatica. However, the specific anti-neuralgia mechanism of Stauntonia chinensis is unknown. In this study, the analgesic effect of Stauntonia chinensis injection (SCI) in mice with neuropathic pain and the possible mechanisms are explored. We find that a local injection of 0.1 mL Stauntonia chinensis for 14 days can considerably relieve mechanical hyperalgesia and thermal hyperalgesia in mice with sciatic chronic constriction injury (CCI). Immunofluorescence staining shows that SCI reduces neuroinflammation in the spinal cord of CCI mice. RNA sequencing reveals that the expression of postsynaptic density protein 95 (PSD-95), a postsynaptic scaffold protein, is downregulated in the spinal cord of CCI mice, but upregulated after SCI administration. Immunofluorescence experiments also demonstrate that SCI administration reverses microglia proliferation and PSD-95 downregulation in CCI mice. These data suggest that SCI relieves neuropathic pain by increasing the expression of PSD-95 and reducing the proliferation of phagocytic microglia.

Citing Articles

Stauntonia chinensis injection relieves neuropathic pain by increasing the expression of PSD-95 and reducing the proliferation of phagocytic microglia.

Deng W, Zou H, Qian L, de Souza S, Chen Q, Cao S Ibrain. 2024; 10(1):3-18.

PMID: 38682013 PMC: 11045182. DOI: 10.1002/ibra.12140.

References

Yuan S, Ji G, Li B, Andersson T, Neugebauer V, Tang S . A Wnt5a signaling pathway in the pathogenesis of HIV-1 gp120-induced pain. Pain. 2015; 156(7):1311-1319. PMC: 4682897. DOI: 10.1097/j.pain.0000000000000177. View

Bai K, Chuang K, Chen C, Jhan M, Hsiao T, Cheng T . Microglial activation and inflammation caused by traffic-related particulate matter. Chem Biol Interact. 2019; 311:108762. DOI: 10.1016/j.cbi.2019.108762. View

Ji R, Nackley A, Huh Y, Terrando N, Maixner W . Neuroinflammation and Central Sensitization in Chronic and Widespread Pain. Anesthesiology. 2018; 129(2):343-366. PMC: 6051899. DOI: 10.1097/ALN.0000000000002130. View

Yousefpour N, Locke S, Deamond H, Wang C, Marques L, St-Louis M . Time-dependent and selective microglia-mediated removal of spinal synapses in neuropathic pain. Cell Rep. 2023; 42(1):112010. DOI: 10.1016/j.celrep.2023.112010. View

Jia Y, Zhang D, Yin H, Li H, Du J, Bao H . Ganoderic Acid A Attenuates LPS-Induced Neuroinflammation in BV2 Microglia by Activating Farnesoid X Receptor. Neurochem Res. 2021; 46(7):1725-1736. PMC: 8187184. DOI: 10.1007/s11064-021-03303-3. View

Kawaguchi M, Satoh Y, Otsubo Y, Kazama T . Molecular hydrogen attenuates neuropathic pain in mice. PLoS One. 2014; 9(6):e100352. PMC: 4062523. DOI: 10.1371/journal.pone.0100352. View

Liu Y, Zhou L, Wang J, Li D, Ren W, Peng J . TNF-α Differentially Regulates Synaptic Plasticity in the Hippocampus and Spinal Cord by Microglia-Dependent Mechanisms after Peripheral Nerve Injury. J Neurosci. 2017; 37(4):871-881. PMC: 5296781. DOI: 10.1523/JNEUROSCI.2235-16.2016. View

van Hecke O, Austin S, Khan R, Smith B, Torrance N . Neuropathic pain in the general population: a systematic review of epidemiological studies. Pain. 2013; 155(4):654-662. DOI: 10.1016/j.pain.2013.11.013. View

Luo C, Kuner T, Kuner R . Synaptic plasticity in pathological pain. Trends Neurosci. 2014; 37(6):343-55. DOI: 10.1016/j.tins.2014.04.002. View

10.

Li J, Wei Y, Zhou J, Zou H, Ma L, Liu C . Activation of locus coeruleus-spinal cord noradrenergic neurons alleviates neuropathic pain in mice via reducing neuroinflammation from astrocytes and microglia in spinal dorsal horn. J Neuroinflammation. 2022; 19(1):123. PMC: 9145151. DOI: 10.1186/s12974-022-02489-9. View

11.

Cohen S, Mao J . Neuropathic pain: mechanisms and their clinical implications. BMJ. 2014; 348:f7656. DOI: 10.1136/bmj.f7656. View

12.

Chen S, Rong Y, Liu M, Cheng S, Liu X, Li X . Analgesic Effects of Triterpenoid Saponins From via Selective Increase in Inhibitory Synaptic Response in Mouse Cortical Neurons. Front Pharmacol. 2018; 9:1302. PMC: 6241161. DOI: 10.3389/fphar.2018.01302. View

13.

Gao H, Zhao F, Chen G, Chen S, Yu Y, Yao Z . Bidesmoside triterpenoid glycosides from Stauntonia chinensis and relationship to anti-inflammation. Phytochemistry. 2009; 70(6):795-806. DOI: 10.1016/j.phytochem.2009.04.005. View

14.

Cao S, Fisher D, Rodriguez G, Yu T, Dong H . Comparisons of neuroinflammation, microglial activation, and degeneration of the locus coeruleus-norepinephrine system in APP/PS1 and aging mice. J Neuroinflammation. 2021; 18(1):10. PMC: 7789762. DOI: 10.1186/s12974-020-02054-2. View

15.

Costigan M, Scholz J, Woolf C . Neuropathic pain: a maladaptive response of the nervous system to damage. Annu Rev Neurosci. 2009; 32:1-32. PMC: 2768555. DOI: 10.1146/annurev.neuro.051508.135531. View

16.

Liu S, Chuang W, Lam W, Jiang Z, Cheng Y . Safety surveillance of traditional Chinese medicine: current and future. Drug Saf. 2015; 38(2):117-28. PMC: 4348117. DOI: 10.1007/s40264-014-0250-z. View

17.

Deng W, Zou H, Qian L, de Souza S, Chen Q, Cao S . Stauntonia chinensis injection relieves neuropathic pain by increasing the expression of PSD-95 and reducing the proliferation of phagocytic microglia. Ibrain. 2024; 10(1):3-18. PMC: 11045182. DOI: 10.1002/ibra.12140. View

18.

Jiang Y, Li K, Li X, Xu L, Yang Z . Sodium butyrate ameliorates the impairment of synaptic plasticity by inhibiting the neuroinflammation in 5XFAD mice. Chem Biol Interact. 2021; 341:109452. DOI: 10.1016/j.cbi.2021.109452. View

19.

Chen G, Park C, Xie R, Berta T, Nedergaard M, Ji R . Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice. Brain. 2014; 137(Pt 8):2193-209. PMC: 4107738. DOI: 10.1093/brain/awu140. View

20.

Yang L, Xin X, Zhang J, Zhang L, Dong Y, Zhang Y . Inflammatory pain may induce cognitive impairment through an interlukin-6-dependent and postsynaptic density-95-associated mechanism. Anesth Analg. 2014; 119(2):471-480. PMC: 4107025. DOI: 10.1213/ANE.0000000000000279. View