Green Synthesis of Silver Nanoparticles Using Extract Modified by Chitosan/Alginate: Bactericidal Activity Against Pathogenic Bacteria and Cytotoxicity Analysis in Normal Cell Line

Overview

Journal Curr Pharm Des

Publisher Bentham Science Publishers

Date 2024 Apr 25

PMID 38661036

Authors

Mahnaz Mohtashami

Alieh Rezagholizade-Shirvan

Zahra Hojati Bonab

Mohammad Reza Amiryousefi

Majid Darroudi

Mobina Sadat Ahmadi Solimani

Sajad Yaghoobi

Samaneh Dolatabadi

Ahmad Ghasemi

Amir Abbas Momtazi-Borojeni

Affiliations

Soon will be listed here.

Abstract

Aim: The study aimed to determine pharmacological effects of modified Ag nanoparticles (AgNPs).

Background: AgNPs are considered antimicrobial agents. However, the cytotoxicity of chemically synthesized AgNPs (cAgNPs) has raised challenges that limit their use.

Objective: The purpose of the study was to examine the antimicrobial and cytotoxicity effects of AgNPs synthesized using extract modified by chitosan/alginate AgNPS (Ch/ALG-gAgNPs).

Methods: Nanoparticles were characterized using TEM, DLS, XRD, and FTIR. Resistant strains of were used for the antimicrobial analysis of Ch/ALG-gAgNPs using disc diffusion and microdilution methods. The effects of NPs on cell viability and apoptosis in L929 normal cells were determined using MTT assay and annexin/PI staining, respectively.

Results: Physicochemical characterizations confirmed Ch/ALG-gAgNPs to be spherical and uniformly dispersed, and their size ranged from 50 to 500 nm. Ch/ALG-gAgNPs inhibited the growth of microbial strains in a dose-dependent manner. The antibacterial effect of Ch/ALG-gAgNPs was significantly higher than cAgNPs. The Ch/ALG-gAgNPs showed little cytotoxicity against normal cells at concentrations less than 50 μg/ml. Cytotoxicity effects of Ch/ALG-gAgNP were less than cAgNPs. Flow cytometry and real-time PCR results showed a decrease in apoptosis percentage and BAX marker in the presence of Ch/ALG-gAgNPs relative to when the cell was treated with cAgNPs.

Conclusion: Current findings introduce novel gAgNPs modified with chitosan/alginate for use in medicine.

Citing Articles

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Application of nanomaterials for determination and removal of polycyclic aromatic hydrocarbons in food products: A review.

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