The Chromatin-associated 53BP1 Ortholog, HSR-9, Regulates Recombinational Repair and Chromosome Segregation in the Germ Line

Overview

Journal bioRxiv

Date 2024 Apr 25

PMID 38659880

Authors

Qianyan Li

Sara Hariri

Aashna Calidas

Arshdeep Kaur

Erica Huey

JoAnne Engebrecht

Affiliations

Soon will be listed here.

Abstract

53BP1 plays a crucial role in regulating DNA damage repair pathway choice and checkpoint signaling in somatic cells; however, its role in meiosis has remained enigmatic. In this study, we demonstrate that the ortholog of 53BP1, HSR-9, associates with chromatin in both proliferating and meiotic germ cells. Notably, HSR-9 is enriched on the chromosome pair in pachytene oogenic germ cells. HSR-9 is also present at kinetochores during both mitotic and meiotic divisions but does not appear to be essential for monitoring microtubule-kinetochore attachments or tension. Using cytological markers of different steps in recombinational repair, we found that HSR-9 influences the processing of a subset of meiotic double strand breaks into COSA-1-marked crossovers. Additionally, HSR-9 plays a role in meiotic chromosome segregation under conditions where chromosomes fail to pair, synapse, and recombine. Together, these results highlight that chromatin-associated HSR-9 has both conserved and unique functions in the regulation of meiotic chromosome behavior.

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