In Vitro Photobiomodulation Effects of Blue and Red Diode Lasers on Proliferation and Differentiation of Periodontal Ligament Mesenchymal Stem Cells

Overview

Journal J Lasers Med Sci

Date 2024 Apr 24

PMID 38655041

Authors

Ferena Sayar

Ahmad Garebigloo

Sogol Saberi

Ardavan Etemadi

Affiliations

Soon will be listed here.

Abstract

This study aimed to assess the photobiomodulation effects of blue and red lasers on the proliferation and osteogenic differentiation of periodontal ligament mesenchymal stem cells (PDLMSCs). PDLMSCs were cultured and tested in 4 groups. The first two groups were exposed to 445 nm diode laser irradiation (200 mW, 6 and 12 J/cm ), and the third group was exposed to 660 nm diode laser irradiation (50 mW, 4 J/cm ). The fourth group was also considered as the control group without irradiation. Cell viability/proliferation was assessed by MTT assay. RUNX2, alkaline phosphatase (ALP), collagen type 1 (col1), and osteocalcin (OCN) were evaluated by RT-PCR, and Alizarin red was used to evaluate the colonization. The data were analyzed by means of one-way analysis of variance. The results of our study showed that cell survival/proliferation in the second group was significantly lower than that in the control group on days 1 and 7 (<0.05). RT-PCR showed a significant increase in osteogenic genes in all three laser groups compared to the control group (<0.05). All groups showed a significant increase in calcium content compared to the control group (<0.05). ALP activity also confirmed the osteoblastic differentiation of cells in laser groups. 445 nm and 660 nm lasers with the studied parameters showed positive effects on the proliferation and osteoblastic differentiation of PDLMSCs.

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