Preparation of β(1→3)/β(1→4) Xylooligosaccharides from Red Alga Dulse by Two Xylanases from Streptomyces Thermogriseus

Overview

Journal Bioresour Bioprocess

Specialty Biochemistry

Date 2024 Apr 23

PMID 38650209

Authors

Yuki Fujii

Manami Kobayashi

Yoshikatsu Miyabe

Hideki Kishimura

Tadashi Hatanaka

Yuya Kumagai

Affiliations

Soon will be listed here.

Abstract

Red alga dulse contains xylan with β(1→3)/β(1→4) linkages. We previously prepared xylooligosaccharides (XOSs) from dulse xylan; however, the product contained many D-xylose residues and fewer XOSs with β(1→3) linkages. To improve the efficiency of XOS production, we prepared two recombinant endoxylanases from Streptomyces thermogriseus (StXyl10 and StXyl11). Comparing the k/K values for dulse xylan, this value from StXyl10 was approximately two times higher than that from StXyl11. We then determined the suitable conditions for XOS production. As a result, dulse XOS was prepared by the successive hydrolysis of 10 mg/mL dulse xylan by 0.5 μg/mL StXyl10 for 4 h at 50 °C and then 2.0 μg/mL StXyl11 for 36 h at 60 °C. Xylan was converted into 95.8% XOS, including 59.7% XOS with a β(1→3) linkage and 0.97% D-xylose. Our study provides useful information for the production of XOSs with β(1→3) linkages.

Citing Articles

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