The Potential Molecular Mechanism Underlying Gypenoside Amelioration of Atherosclerosis in ApoE Mice: A Multi-omics Investigation

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Apr 22

PMID 38644881

Authors

Xing Ju

Yufeng Liu

Ying Wang

Guoyuan Sui

Yixin Ma

Huimin Cao

Yuan Cao

Jin Wu

Ying Du

Xue Leng

Lianqun Jia

Guanlin Yang

Affiliations

Soon will be listed here.

Abstract

Gypenosides (Gyp) are bioactive components of that have a variety of pharmacological properties. Extracts of have been found to be effective in the reduction of blood sugar and lipids and prevention of atherosclerosis. Here, the functions of Gyp and the mechanisms underlying their effects on atherosclerosis were investigated. Mice were allocated to three groups, namely, the control (C57BL/6), atherosclerosis model (ApoE mice with high-fat diet), and Gyp-treated groups. Differentially expressed mRNAs, miRNAs, circRNA, and differential metabolites among the groups were analyzed. The results showed that "Fatty acid metabolism", "Fatty acid elongation", "Cytokine-cytokine receptor interaction", and "PI3K-Akt signaling pathway", amongst others, were involved in treatment process. Differentially expressed genes, including , , and were also identified. Mmu-miR-30a and mmu-miR-30e showed reduced expression in atherosclerosis models but were increased following Gyp treatment, suggesting involvement in the effects of Gyp. In addition, chr5:150604177-150608440 were found to interact with mmu-miR-30a and mmu-miR-30e to regulate their abundance. In terms of metabolomics, Gyp may regulate biological processes involving PGD and PGJ, potentially alleviating atherosclerosis. In conclusion, Gyp appeared to have complex effects on atherosclerosis, most of which were positive. These results support the use of Gyp in the treatment of atherosclerosis.

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