CD14+ Dendritic-Shaped Cells Functioning As Dendritic Cells in Rheumatoid Arthritis Synovial Tissues

Overview

Journal ACR Open Rheumatol

Date 2024 Apr 19

PMID 38638058

Authors

Rie Kurose

Takashi Satoh

Akira Kurose

Yasuyuki Ishibashi

Miwa Uzuki

Yuji Wakai

Tomoyuki Sasaki

Kinji Ishida

Katsutoshi Ogasawara

Takashi Sawai

Affiliations

Soon will be listed here.

Abstract

Objective: We previously reported that CD14+ dendritic-shaped cells exhibit a dendritic morphology, engage in pseudo-emperipolesis with lymphocytes, and express CD90 in the perivascular areas of rheumatoid arthritis (RA) synovial tissues. However, it remains unclear whether these CD14CD90 cells function as dendritic cells. In this study, we investigated the dendritic cell-differentiation potential of CD14CD90 cells.

Methods: The localization and number of CD14CD90 cells in RA synovial tissues and peripheral blood were examined. The dendritic cell-differentiation potential of CD14CD90 cells was examined by measuring interleukin-6 and tumor necrosis factor-α levels in the supernatant and CD83 and human leukocyte antigen (HLA)-DR expression in the cells after induction of dendritic cell differentiation. Synovial cells were co-cultured with lymphocytes, and the activation of these cells was examined.

Results: CD14CD90 cells were abundant in RA synovial tissues, including the sublining layer and the pannus areas. Patients with untreated and active RA had significantly higher percentages of CD14CD90 cells in the peripheral blood and synovial tissues. In RA synovial cells, inflammatory cytokine levels increased with dendritic cell-differentiation culture, but CD83 and HLA-DR expression were significantly increased in the CD14CD90 cell group. When co-cultured with lymphocytes, cell numbers and inflammatory cytokine levels significantly increased in both groups of synovial cells after dendritic cell induction.

Conclusion: CD14+ cells migrate and spread from the circulating blood to RA synovial tissues while expressing CD90, and CD14CD90 cells in contact with lymphocytes differentiate into HLA-DR+ dendritic cells, which contribute to chronic inflammation in RA.

Citing Articles

Mendelian randomization and mediation analysis reveal the role of immune cell subsets in the causal pathways between blood cell perturbation responses and rheumatoid arthritis.

Li F, Xian D, Yang K Clin Rheumatol. 2025; .

PMID: 40072781 DOI: 10.1007/s10067-025-07387-y.

Correction to "CD14+ Dendritic-Shaped Cells Functioning as Dendritic Cells in Rheumatoid Arthritis Synovial Tissues".

ACR Open Rheumatol. 2025; 7(1):e11777.

PMID: 39749998 PMC: 11697260. DOI: 10.1002/acr2.11777.

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