Characterization of the Gut Bacterial and Viral Microbiota in Latent Autoimmune Diabetes in Adults

Overview

Journal Sci Rep

Specialty Science

Date 2024 Apr 9

PMID 38594375

Authors

Casper S Poulsen

Dan Hesse

Gabriel R Fernandes

Tue H Hansen

Timo Kern

Allan Linneberg

Lore Van Espen

Torben Jorgensen

Trine Nielsen

Amra C Alibegovic

Jelle Matthijnssens

Oluf Pedersen

Henrik Vestergaard

Torben Hansen

Mette K Andersen

Affiliations

Soon will be listed here.

Abstract

Latent autoimmune diabetes in adults (LADA) is a heterogeneous disease characterized by autoantibodies against insulin producing pancreatic beta cells and initial lack of need for insulin treatment. The aim of the present study was to investigate if individuals with LADA have an altered gut microbiota relative to non-diabetic control subjects, individuals with type 1 diabetes (T1D), and individuals with type 2 diabetes (T2D). Bacterial community profiling was performed with primers targeting the variable region 4 of the 16S rRNA gene and sequenced. Amplicon sequence variants (ASVs) were generated with DADA2 and annotated to the SILVA database. The gut virome was sequenced, using a viral particle enrichment and metagenomics approach, assembled, and quantified to describe the composition of the viral community. Comparison of the bacterial alpha- and beta-diversity measures revealed that the gut bacteriome of individuals with LADA resembled that of individuals with T2D. Yet, specific genera were found to differ in abundance in individuals with LADA compared with T1D and T2D, indicating that LADA has unique taxonomical features. The virome composition reflected the stability of the most dominant order Caudovirales and the families Siphoviridae, Podoviridae, and Inoviridae, and the dominant family Microviridae. Further studies are needed to confirm these findings.

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