The Deubiquitinase Cofactor UAF1 Interacts with USP1 and Plays an Essential Role in Spermiogenesis

Overview

Journal iScience

Publisher Cell Press

Date 2024 Apr 9

PMID 38591005

Authors

Ziqi Wang

Tongtong Li

Dongkai Liu

Mengjing Li

Shangming Liu

Xiaochen Yu

Hanzhen Li

Hui Song

Wei Zhao

Zhaojian Liu

Xiangfeng Chen

Gang Lu

Zi-Jiang Chen

Tao Huang

Hongbin Liu

Affiliations

Soon will be listed here.

Abstract

Spermiogenesis defines the final phase of male germ cell differentiation. While multiple deubiquitinating enzymes have been linked to spermiogenesis, the impacts of deubiquitination on spermiogenesis remain poorly characterized. Here, we investigated the function of UAF1 in mouse spermiogenesis. We selectively deleted in premeiotic germ cells using the knock-in mouse strain ( sKO), and found that is essential for spermiogenesis and male fertility. Further, UAF1 interacts and colocalizes with USP1 in the testes. Conditional knockout of in testes results in disturbed protein levels and localization of USP1, suggesting that UAF1 regulates spermiogenesis through the function of the deubiquitinating enzyme USP1. Using tandem mass tag-based proteomics, we identified that conditional knockout of in the testes results in reduced levels of proteins that are essential for spermiogenesis. Thus, we conclude that the UAF1/USP1 deubiquitinase complex is essential for normal spermiogenesis by regulating the levels of spermiogenesis-related proteins.

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