Malondialdehyde Assays in Higher Plants

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Apr 8

PMID 38587737

Authors

Melanie Morales

Sergi Munne-Bosch

Affiliations

Soon will be listed here.

Abstract

Malondialdehyde is a three-carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation widely used as a marker of the extent of lipid peroxidation in plants. There are several methodological approaches to quantify malondialdehyde contents in higher plants, ranging from the simplest, cheapest, and quickest spectrophotometric approaches to the more complex ones using tandem mass spectrometry. This chapter summarizes the advantages and limitations of approaches followed and provides brief protocols with some tips to facilitate the selection of the best method for each experimental condition and application.

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References

Farmer E, Mueller M . ROS-mediated lipid peroxidation and RES-activated signaling. Annu Rev Plant Biol. 2013; 64:429-50. DOI: 10.1146/annurev-arplant-050312-120132. View

Sofo A, Dichio B, Xiloyannis C, Masia A . Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress. Physiol Plant. 2004; 121(1):58-65. DOI: 10.1111/j.0031-9317.2004.00294.x. View

Weber H, Chetelat A, Reymond P, Farmer E . Selective and powerful stress gene expression in Arabidopsis in response to malondialdehyde. Plant J. 2004; 37(6):877-88. DOI: 10.1111/j.1365-313x.2003.02013.x. View

Heath R, Packer L . Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968; 125(1):189-98. DOI: 10.1016/0003-9861(68)90654-1. View

Taulavuori E, Hellstrom E, Taulavuori K, Laine K . Comparison of two methods used to analyse lipid peroxidation from Vaccinium myrtillus (L.) during snow removal, reacclimation and cold acclimation. J Exp Bot. 2001; 52(365):2375-80. DOI: 10.1093/jexbot/52.365.2375. View

Davey M, Stals E, Panis B, Keulemans J, Swennen R . High-throughput determination of malondialdehyde in plant tissues. Anal Biochem. 2005; 347(2):201-7. DOI: 10.1016/j.ab.2005.09.041. View

Yonny M, Rodriguez Torressi A, Nazareno M, Cerutti S . Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry. J Anal Methods Chem. 2017; 2017:4327954. PMC: 5288533. DOI: 10.1155/2017/4327954. View

Lykkesfeldt J . Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible spectrophotometry. Clin Chem. 2001; 47(9):1725-7. View

10.

Munne-Bosch S, Alegre L . Interplay between ascorbic acid and lipophilic antioxidant defences in chloroplasts of water-stressed Arabidopsis plants. FEBS Lett. 2002; 524(1-3):145-8. DOI: 10.1016/s0014-5793(02)03041-7. View

11.

Draper H, Squires E, Mahmoodi H, Wu J, Agarwal S, Hadley M . A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med. 1993; 15(4):353-63. DOI: 10.1016/0891-5849(93)90035-s. View

12.

Deighton N, Magill W, Bremner D, Benson E . Malondialdehyde and 4-hydroxy-2-nonenal in plant tissue cultures: LC-MS determination of 2,4-dinitrophenylhydrazone derivatives. Free Radic Res. 1997; 27(3):255-65. DOI: 10.3109/10715769709065764. View

13.

Deighton , Muckenschnabel I , GOODMAN , Williamson . Lipid peroxidation and the oxidative burst associated with infection of capsicum annuum by botrytis cinerea. Plant J. 1999; 20(4):485-92. DOI: 10.1046/j.1365-313x.1999.00622.x. View

14.

Muckenschnabel I, Williamson B, Goodman B, Lyon G, Stewart D, Deighton N . Markers for oxidative stress associated with soft rots in French beans (Phaseolus vulgaris) infected by Botrytis cinerea. Planta. 2001; 212(3):376-81. DOI: 10.1007/s004250000401. View

15.

Yeo H, Helbock H, Chyu D, Ames B . Assay of malondialdehyde in biological fluids by gas chromatography-mass spectrometry. Anal Biochem. 1994; 220(2):391-6. DOI: 10.1006/abio.1994.1355. View